Tobias Baskin's sort-of real time science blog
I am fretful. Forgetful too, but that is a story for elsewhere. Last week, my efforts to find out what is going wrong have shown me things look very wrong. I don’t know why; I am unsure what to do next. I have appealed to my expert colleagues, Rudolf Oldenbourg and Amit Verma of Marine […]
Pinholes and pinheads! Before getting to the latest installment of the Calibration Campaign, I want to remind everyone that I welcome questions and comments (unless you are a troll!). Happy to answer. Also do hit the subscribe button so you never miss a post; and please tell your friends! With that, on to the latest. […]
Last week, I wrote about my misgivings over the 5 degrees of separation (actually, of misalignment) between the rotatable polarizer’s positioning on the microscope stage and the polarization of the microscope’s laser. In discussing with Rudolf Oldenbourg (MBL), I learned that the misalignment does not matter. What does matter is that the peaks and valleys […]
Last week, I carried on the quest for calibration. Avalon remains stubbornly out of reach. I tried things I mentioned last week and a new thing besides. The liquid crystal device seems undamaged. To have a look, I improvised a light-table next to the confocal microscope. I put a flashlight inside a jar (otherwise used […]
I can think of stronger words: mutilation? defenestration? OK, OK. I was not injured let alone thrown out of a window. This past week, for the first time, I tried to calibrate the system. What system? The one I brought here to observe and quantify polarized fluorescence on a confocal microscope. The heart of the […]
Maestro to the pit; actors to their places; its time to raise the curtain. Sure, that might be happening over at the Crescent Theatre with their latest production, but I am talking about mine: I am ready to collect data. I am ready because of wo breakthroughs that happened in January. OK, the word breakthrough implies fabulous […]
Happy 2024! I hope your year is full of peace and projects. And of course, lots of blogs! Maybe some art too (Fig. 1). On my last post, I described how I plan to observe the orientation of cellulose in the root epidermis. To recap, I need to stain cellulose with a fluorescent dye and […]
At least one rabbit anyway. About a month ago, I wrote about falling down a hole chasing not a rabbits but root hairs. In efforts to coax fast scarlet into staining roots uniformly, I incorporated the dye into the agar medium, inside of which the roots grow. Alas, giving the roots continuous exposure failed to […]
… bit me. Of course, they always do. Examining an assumption reveals either that it is ok, to a first approximation, or that it isn’t; in the first case, the conditions are accepted as a reasonable compromise and, in the second case, conditions are changed. Without any check, unfortunate conditions come along for the ride, […]
I want to make roots twist more vigorously; instead, I found a way to make twisting almost stop. As I have written about earlier (here and here), root twisting in my trials has been somewhat … flakey. I brought seed of six genotypes of lab weed (aka arabidopsis) that are recognized as twisting mutants. These […]