Mysterio-plasmic reticulum

No post last week because my wife Laura and I had spent the week before in Rome. I considered blogging about our sightseeing, we did visit the Botanical Gardens—well worth a tourist’s digression, but I felt that travel-gazing is not quite the spirit of Lab Fab. Also, I was flat-out tired: visiting Rome features kilometers and […]

A quiet interlude

A short post today. For one thing, I just spent an hour dealing with the reservation for Laura and my stay in Rome, Italy, being peremptorily cancelled. We are supposed to check in tomorrow. We got an email this afternoon from the quaint B&B “Livia” saying sorry you cannot stay with us, our toilet has […]

A himmer of glope

March 31 is International Transgender Day of Visibility (Fig. 1). Please do something nice for your trans friends today (and everyday). As for the title of today’s post, maybe it is the other way around? Last week, I was shut out from tweaking the calibration of the liquid crystals (discussed here) because the software glitch […]

The pinhole test

Pinholes and pinheads! Before getting to the latest installment of the Calibration Campaign, I want to remind everyone that I welcome questions and comments (unless you are a troll!). Happy to answer. Also do hit the subscribe button so you never miss a post; and please tell your friends!  With that, on to the latest. […]

And then there was biology

Last week, I wrote about my misgivings over the 5 degrees of separation (actually, of misalignment) between the rotatable polarizer’s positioning on the microscope stage and the polarization of the microscope’s laser. In discussing with Rudolf Oldenbourg (MBL), I learned that the misalignment does not matter. What does matter is that the peaks and valleys […]

Five degrees of separation

Last week, I carried on the quest for calibration. Avalon remains stubbornly out of reach. I tried things I mentioned last week and a new thing besides. The liquid crystal device seems undamaged. To have a look, I improvised a light-table next to the confocal microscope. I put a flashlight inside a jar (otherwise used […]

Calibration consternation

I can think of stronger words: mutilation? defenestration? OK, OK. I was not injured let alone thrown out of a window.  This past week, for the first time, I tried to calibrate the system. What system? The one I brought here to observe and quantify polarized fluorescence on a confocal microscope. The heart of the […]

Show time

Maestro to the pit; actors to their places; its time to raise the curtain. Sure, that might be happening over at the Crescent Theatre with their latest production, but I am talking about mine: I am ready to collect data.  I am ready because of wo breakthroughs that happened in January. OK, the word breakthrough implies fabulous […]

Another way to image cellulose?

Happy 2024! I hope your year is full of peace and projects. And of course, lots of blogs!  Maybe some art too (Fig. 1).  On my last post, I described how I plan to observe the orientation of cellulose in the root epidermis. To recap, I need to stain cellulose with a fluorescent dye and […]