*denotes corresponding authorship
2024
Pavon N†, Diep K†, Yang F, Sebastian R†, Martinez-Martin B†, Ranjan R, Sun Y*, Pak C*. “Patterning ganglionic eminences in developing human brain organoids using morphogen induced gradient device.” Cell Reports Methods. 2024 Jan 22;4(1):100689.doi: 10.1016/j.crmeth.2023.100689. PMID: 38228151
2023
Pavon N†, Diep K†, Yang F, Sebastian R†, Martinez-Martin B†, Ranjan R, Sun Y*, Pak C*. “Patterning ganglionic eminences in developing human brain organoids using morphogen induced gradient device.” [bioRxiv preprint – https://www.biorxiv.org/content/10.1101/2023.06.20.545705v1]
Sebastian R†, Jin K, Pavon N†, Bansal R†, Potter A, Song Y†, Babu J†, Gabriel R†, Sun Y, Aronow B, Pak C*. “Schizophrenia-associated NRXN1 deletions induce developmental-timing- and cell-type-specific vulnerabilities in human brain organoids.” Nature Communications. 2023 Jun 24;14(1):3770. doi: 10.1038/s41467-023-39420-6. PMID: 37355690
Dawes P, Smullen M, Fernandez-Fontaine A, Zhang Y, English J†, Uddin M, Pak C, Church GM, Chan Y, Lim ET. “oFlowSeq: A multiplexed quantitative approach to identify genes affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoids.” Human Genetics. 2023 In press.PMID: 36877372
English J†, McSweeney D†, Howell E†, Ribbe F†, Pak C*. 2023. “Generation and Co-culture of Cortical Glutamatergic and GABAergic-Induced Neuronal Cells.” In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY.
Sebastian R†, Pavon N†, Diep K†, Song Y†, Pak C*. 2023. “Method to generate patterned forebrain organoids from iPSCs.” In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY.
McSweeney D†, English J†, Howell E†, Ribbe F†, Pak C*. 2023. “Measuring neuronal network activity using induced neuronal cells.” In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY.
2022
Xie T, Brown LE, Pak C, Sun Y. “Self-organized anteroposterior regionalization of early midbrain and hindbrain using micropatterned human embryonic stem cells.” [bioRxiv preprint – https://www.biorxiv.org/content/10.1101/2022.07.22.501065v1]
Sebastian R†, Jin K, Pavon N†, Bansal R†, Potter A, Song Y†, Babu J†, Gabriel R†, Sun Y, Aronow B, Pak C*. “Single cell transcriptomic profiles of developing brain organoids reveal time- and cell-type specific vulnerabilities of NRXN1 CNVs in schizophrenia.” [bioRxiv preprint – https://www.biorxiv.org/content/10.1101/2022.08.24.505165v1]
Wang L, Mirabella VR, Dai R, Su X, Xu R, Jadali A, Bernabucci M, Singh I, Chen Y, Tian Y, Jiang P, Kwan KY, Pak C, Liu C, Comoletti D, Hart RP, Chen C, Sudhof TC, Pang ZP. “Analyses of the autism-associated Neuroligin-3 R451C mutation in human neurons reveals a gain-of-function synaptic mechanism.” Molecular Psychiatry. 2022 Oct 24. doi: 10.1038/s41380-022-01834-x. Online ahead of print. PMID: 36280753
McSweeney D†, Gabriel R†, Jin K, Pang ZP, Aronow B, Pak C*. “CASK loss-of-function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons” iScience 2022 Sep 23;25(10):105187. PMID: 36262316.
Sebastian R†, Song Y†, Pak C*. “Probing the molecular and cellular pathological mechanisms of schizophrenia using human induced pluripotent stem cell models.” Schizophrenia Research. 2022 Jul 11;S0920-9964(22)00263-8. PMID: 35835709
2021
Pak C*, Sun Y*. “Organoids: expanding applications enabled by emerging technologies.” Editorial Review. Special issue on “Organoids,” Journal of Molecular Biology. 2021 Dec 20;167411. Online ahead of print. PMID: 34933020
Pak C*, Danko T, Mirabella V, Wang J, Liu Y, Vangipuram M, Grieder S, Zhang X, Ward T, Huang A, Jin K, Dexheimer P, Bardes E, Mittelpunkt A, Ma J, McLachlan M, Moore JC, Qu P, Purmann C, Dage JL, Swanson BJ, Urban AE, Aronow BJ, Pang ZP, Levinson DF, Wernig M, Südhof TC*. “Cross-platform validation of neurotransmitter release impairments in schizophrenia patient-derived NRXN1-mutant neurons.” PNAS 2021, Jun 1;118(22):e2025598118. PMID: 34035170
Fuccillo MV*, Pak C*. “Copy number variants in neurexin genes: phenotypes and mechanisms.” Current Opinion in Genetics and Development. 2021 Mar20:68:64-70. PMID: 33756113
Li N, Yang F, Parthasarathy S, St. Pierre S, Hong K, Pavon N†, Pak C, Sun Y. “Patterning Neuroepithelial Cell Sheet via a Sustained Chemical Gradient Generated by Localized Passive Diffusion Devices.” ACS Biomaterial Science Engineering. 2021, Apr 12;7(4):1713-1721. PMID: 33751893
2020
Xie T, Kang J, Pak C, Yuan H, Sun Y. “Temporal modulations of NODAL, BMP and WNT signals guide the spatial patterning in self-organized human ectoderm tissues.” Matter 2020, 2(6) June:1621-1638. “PMC Journal in process”
Galarza S, Crosby AJ, Pak C, Peyton SR. “Control of Astrocyte Quiescence and Activation in a Synthetic Brain Hydrogel.” Advanced Healthcare Materials 2020, 9(4):31901419 PMID: 31943839
Prior to 2018
Pak C, Grieder S, Yang N, Zhang Y, Wernig M, Südhof TC. “Rapid generation of functional and homogeneous excitatory human forebrain neurons using Neurogenin-2 (Ngn2),” Nature Protocol Exchange 2018, DOI:.10.1038/protex.2018.082.
Bienkowski R, Rha J, Banerjee A, Rounds JC, Gross C, Pak C, Morris KJ, Jones SK, Santoro MR, Warren ST, Bassell GJ, Corbett AH, Moberg KH. “The conserved, disease-assciated RNA-binding protein dNab2 interacts with the Fragile-X protein ortholog in Drosophila neurons.” Cell Reports 2017, 20(6)1372-1384 PMID: 28793261
Lee SJ, Wei M, Zhang C, Maxeiner S, Pak C, Botelho SC, Trotter J, Sterky FH, Südhof TC. “Presynaptic neuronal pentraxin receptor organizes excitatory and inhibitory synapses.” Journal of Neuroscience 2016, 2768-16 PMID: 27986928
Fei Y, Danko, Botelho SB, Patzke, Pak C, Wernig M, Südhof TC, “Autism-Associated SHANK3 Haploinsufficiency Causes Ih-Channelopathy in Human Neurons.” Science 2016 352 (6286): aaf2669 PMID: 26966193
Pak C, Danko T, Zhang Y, Aoto J, Anderson G, Maxeiner S, Yi F, Wernig M, Südhof TC, “Human neuropsychiatric disease modeling using conditional deletion reveals synaptic transmission defects caused by heterozygous mutations in NRXN1.” Cell Stem Cell 2015 17 (3) 316-328 PMID: 26279266
Chanda S, Ang CE, Davila J, Pak C, Mall M, Lee QY, Ahlenius H, Jung SW, Südhof TC, Wernig M “Generation of induced neuronal cells by the single reprogramming factor ASCL1.” Stem Cell Reports 2014 3 (2) 282-296 PMID: 25254342
Kelly SM, Leung SW, Pak C, Banerjee A, Moberg KH, and Corbett AH, “A conserved role for the zinc finger polyadenosine RNA binding protein, ZC3H14, in control of poly(A) tail length.” RNA 2014 20 1-9 PMID: 24671764
Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, Südhof TC “Rapid single-step induction of functional neurons from human pluripotent stem cells.” Neuron 2013 78 (5) 785-798 PMID: 23764284
Kelly SM, Pak C, Kuss A, Corbett AH, Moberg KH. “New kid on the ID block: Neural functions of the Nab2/ZC3H14 class of Cys3His tandem zinc-finger poly(A)-binding proteins.” Invited Point of View for RNA Biology. RNA Biology 2012 May 1;9(5) PMID:22614829
Pak C, Garshasbi M, Kahrizi M, Gross C, Apponi LH, Noto JJ, Kelly SM, Leung SW, Tzschach A, Behjatie F, Abedinie SS, Mohsenie M, Jensen LR, Hu H, Huang B, Stahley SN, Liu G, Williams KR, Burdick SK, Feng Y, Sanyal S, Bassell GJ, Ropers HH, Najmabadi H, Corbett AH, Moberg KH, Kuss AW. “Mutation of the conserved polyadenosine RNA-binding protein ZC3H14/dNab2 impairs neural function in Drosophila and humans.” PNAS 2011 108 (30) 12390-12395 PMID:21734151
Allan AM, Liang X, Luo Y, Pak C, Li X, Szulwach KE, Chen D, Jin P, Zhao X. “The loss of methyl-CpG binding protein 1 leads to autism-like behavioral deficits.” Human Molecular Genetics 2008 17 (13) 2047-57 PMID:18385101
Zhao X, Pak C, Smrt RD, Jin P. “Epigenetics and Neural developmental disorders: Washington DC, September 18 and 19, 2006.” Epigenetics 2007 2 (2) 126-34 PMID:17965627
Duan R, Pak C, Jin P. “Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA.” Human Molecular Genetics 2007 16 (9) 1124-31 PMID:17400653