Spermidine

Spermidine:

  • neutralizes and stabilizes the negative charge on the DNA phosphate backbone
  • displaces other ions from DNA
  • stimulates restriction enzyme reactions
  • increases specificity and reproducibility of Taq-mediated PCR

Used in Gene & Genome1 M stock20 mM student solutions

To make µL 20mM Spermidine
add: µL 1M spermidine
to: µL water