YEPAD

(Yeast Extract + Peptone + Adenine + Dextrose) contains the same ingredients as YEAD but has peptone added. This is a very rich medium used for storing yeast strains.

To make YEPAD agar plates
at mm plate diameter
pour mL per plate
start with: mL water
add: g yeast extract, stir till dissolved
add: g peptone, stir till dissolved
add: g agar, leave stir bar in
autoclave for: minutes
add asceptically mL filter-sterilized 1 mg/mL adenine
add asceptically mL filter sterilized 20% glucose
for a final volume of mL

Mark with 1 black, 1 red, 1 blue lines