From Useful Numbers for Cell culture

(See also counting cells)

Container	Surface area (cm2)	Seeding density*	Cells at confluency	mL growth medium
35 mm dish	8.8	0.3 x 106	1.2 x 106	2
60 mm dish	21.5	0.8 x 106	3.2 x 106	5
100 mm dish	56.7	2.2 x 106	8.8 x 106	12
150 mm dish	145	5.0 x 106	20.0 x 106	30
6-well plate	9.6	0.3 x 106	1.2 x 106	1 to 3
12-well plate (~20 mm)	3.5	0.1 x 106	0.5 x 106	1 to 2
24-well plate (~15 mm)	1.9	0.05 x 106	0.24 x 106	0.5 to 1.0
48-well plate	1.1	0.03 x 106	0.12 x 106	0.2 to 0.4
96-well plate	0.32	0.01 106	0.04 x 106	0.1 to 0.2
T-12.5 flask	12.5	0.35 x 106	1.4 x 106	1.5-2.5
T-25 flask	25	0.7 x 106	2.8 x 106	3–5
T-75 flask	75	2.1 x 106	8.4 x 106	8–15
T-175 flask	175	4.9 x 106	23.3 x 106	35–53
T-225 flask	225	6.3 x 106	30 x 106	45–68

• Seeding density is given for each culture vessel type as follows:
  • Dishes and Flasks: Cells per vessel;
  • Culture plates: Cells per well

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