Dilution buffer for NEB enzymes
Buffer composition:
| Reagent | Final concentration |
|---|---|
| NaCl | 300 mM |
| Tris-HCl | 10 mM |
| DTT | 1 mM |
| EDTA | 0.1 mM |
| BSA | 500 ug/mL |
| glycerol | 50% |
| pH | 7.4 |
To make 50 mL for use, make 25 mL of 2x, then mix 1:1 with glycerol
25 mL 2x Diluent B
| Reagent | c1 | c2 | v1 |
|---|---|---|---|
| NaCl | 5M | 0.3M | 3 mL |
| Tris HCl | 1M | 0.01M | 0.5 mL |
| DTT | 1 M | 1 mM | 0.05 mL |
| EDTA | 500 mM | 0.1 mM | 0.01 mL |
plus BSA 25 mg
pH to 7.4, bring volume to 25, add glycerol to 50.
Filter sterilize