Nocodazole

Prevents polymerization of microtubules. Prevents cells from entering metaphase.

  • Acros 358240100, 10 mg
  • Soluble in DMSO
  • Dilute stock in medium to treat cells.
  • Make a 10 mg/mL (=33mM) stock: Add 1 mL DMSO to 10 mg in bottle.
  • Aliquot 3 µL 33 mM per tube.
  • Working concentration range is usually 100nM – 30 µM.
  • add 97 µL DMSO to tube to make 100 µL of 1 mM
  • 33 µL 1mM + 10 mL medium = 3.3 µM