Alkaline Lysis Reagent for HotSHOT DNA prep
Recipe from Mike Charles 10/15/03; mikchar@umich.edu; retrieved from Craig Albertson’s lab, recalculated for1 M NaOH – 10 M will damage your plastic tube!!
| Reagent | stock conc | final conc | amt |
|---|---|---|---|
| NaOH | 1 M | 25 mM | 1.25 mL |
| EDTA | 0.5 M | 0.2 mM | 20 uL |
| dI water | to final volume | 50 mL |
Calculator
pH =~12