HeLas for teaching microscopy
MEFs for CURE
| day | date | prep |
|---|---|---|
| Tu | 9/3 | 12 coverslips: HeLa (microtubules, actin, DAPI) |
| Th | 9/5 | (Transmitted light and alignment) |
| Tu | 9/10 | (Cameras, SNR, calibration) |
| Th | 9/12 | (NA and resolution) beads |
| Tu | 9/17 | (Fluorescence) |
| Th | 9/19 | photobleaching (need new slides?) |
| Tu | 9/24 | IF: need fixed coverslips, 10, 20 Ab |
| Th | 9/26 | view IF from previous class, cell culture (MEF 39) |
| Tu | 10/1 | Live cell imaging and cell culture |
| Th | 10/3 | Plasmid prep Eb1 into MEF 39 and cell culture Kate transfects, puts into live dishes |
| Tu | 10/8 | Transfection imaging |
| Th | 10/10 | midterm |
| Tu | 10/15 | NO CLASS (Monday schedule) |
| Th | 10/17 | Begin CURE |
| Tu | 10/22 | need 6 HeLa coverslips for IF rabbit Lamin B1, green mouse Emerin red |
| Th | 10/24 | |
| Tu | 10/29 | |
| Th | 10/31 | |
| Tu | 11/5 | NO CLASS (Election Day) |
| Th | 11/7 | |
| Tu | 11/12 | |
| Th | 11/14 | |
| Tu | 11/19 | |
| Th | 11/21 | |
| Tu | 11/26 | CURE written report due |
| Th | 12/3 | (Michael) Neurobiology |
| Tu | 12/5 | (Michael) Neurobiology |
| Tu | 12/10 | Flexible last day of class |