Gene & Genome Analysis (Bio 383)

class	day	date	topics
1	W	9/3	Tom: Intro to the course Kate: intro to safety, fluorescence microscope live GFP-tubulin S2 cell imaging on 8 conA coverslip dishes (prepared just before class) pipetting part 1
2	M	9/8	pipetting part 2 microscopy HeLa slides S2 culture (24 flasks – each student makes one)
3	W	9/10	Transformation
4	M	9/15	mini prep, pcr
5	W	9/17	0.9% gel, band extraction
6	M	9/22	invitro transcription kit; conA dishes
7	W	9/24	gel, + Nanodrop
8	M	9/29	ds RNA treatment in 2 wells of a 6-well dish
9	W	10/1	16 conA dishes (Tom) 5 mL S2 aliquots
10	M	10/6	IF demo (Kate) repeat ds RNA treatment make 2 conA coverslip per group
11	W	10/8	immunostaining Revised immunostaining for S2 cells IF (students seed coverslips and fix) on control and dsRNA treated cells
	M	10/13	Indigenous People’s Day
12	W	10/15	looking at coverslips (Ctrl vs treated)
13	M	10/20	work on poster presentation
14	W	10/22	presentations (in ILC?)
15	M	10/27	transformations (PXGFP); STLC (dishes); splitting demo
16	W	10/29	Miniprep, nanodrop, restriction digest (Bsb1)
17	M	11/3	gel, gel extraction (1 per student), anneal primers (1 per Group), isothermal reaction, Transformation of Competent Cells 45 min gel rather than 60 min? 15 min, not 15 sec on thermocycler for isothermal rxn split HeLas while gels run
18	W	11/5	transfection of HeLa cells
19	M	11/10
20	W	11/12
21	M	11/17
22	W	11/19
23	M	11/24
	W	11/26	Thanksgiving Break
24	M	12/1
25	W	12/3
26	M	12/8