Zymoclean Gel DNA Recovery Kit Zymo Research D4002

• Add 26 mL 95% EtOH to the 6 mL DNA Wash Buffer concentrate
• Excise DNA fragment (x-tracta Gel Extraction Tool or razor blade)
• Add 3X volume ADB (OR 300 uL ADB/mg gel)
• Incubate 37-55C 5-10 minutes or until gel is dissolved
• transfer melted agarose solution to Zymo Spin Column in a Collection Tube
• centrifuge 10 – 16K Xg 30-60 sec
• add 200 µL DNA Wash Buffer
• Discard flow-through
• Repeat with another 200 µL DNA Wash Buffer. Discard flow-through again
• Add >= 6 µL DNA Elution Buffer (or water)
• Put into clean 1.5 mL tube
• Spin 30-60 sec to elute DNA.

Per rxn:

Reagent	amount	aliquot for 2 rxns	for 3 rxns
ADB1	300 µL	640 µL	950 µL
DNA Wash Buffer	400 µL	840 µL	1250 µL
ENA Elution Buffer	6 µL	15 µL	22 µL (or water?)

• Jewelry scale to weigh gel
• Hot block 55 C for incubation and melting of agarose
• gel excision tools
• 2 mL tube for melting gel
• vortex

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1. volume depends on size of excised gel piece ↩︎