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          • ds RNA treatment
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        • Dog Coat Color
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            • ASIP
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            • TyrP1
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        • mail dog kit
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      • histology
        • Composite Images
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        • Immunofluorescence for histology
        • Paraffin embedding
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        • 2024 Fall Marine Bio
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        • Zebrafish finclip genotyping
      • 2024 Bio Bootcamp
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      • DIY
        • Paper Activities
      • MassBio
      • Middle school 2016
      • Science Quest 2014
      • Summer Genetics
  • Electronics and Equipment
    • 2021 (June) Motic inventory
    • Classroom pipettes
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    • Dissecting Scopes
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    • Fluorescence Microscopes
      • microscope pictures
        • Stage Micrometer images
      • Phase Rings
    • Jenway Genova Spectrophotometer
    • Major equipment
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        • HOBO Data Logger
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      • Programs
      • Script for overnight growth and fluorescence
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    • Suppliers
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      • Thorlabs
  • Protocols
    • Dishwashing
      • Detergent for Dishwasher
    • Drierite Regeneration
    • Husbandry
      • Anemones
        • Exaptasia
        • F/2 Medium for algae (424)
          • Trace minerals for F/2
          • Vitamins for F/2
        • Nematostella
          • Growth chamber spawning program
        • Sea Water
        • Sea Water for 424
        • Symbiodinium
      • Brine Shrimp for Anemones
      • Culturing S-cells
      • E. coli
        • Bacterial media
          • Freezing bacteria
          • Freezing media
          • LB agar
            • LB agar granules
            • LB agar powder
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          • LB antibiotic
          • LB Broth
            • LB broth from mix
            • LB from scratch
            • LB-Amp-Ara
            • LB-sugars
          • MacConkey + bile+ CV
          • MacConkey agar
          • Min med for GFP cells
          • minimal medium (glycerol)
          • Mueller Hinton agar
          • P-Glo Bacterial media
        • BL21(DE3)
      • Mammalian Cell Culture
        • Bulk Coverslip Staining
        • Cell Culture Media
          • DC5 (DMEM for canine cancer)
          • DMEM
          • F10-Ham’s
          • FBS aliquots
          • Freezing Media
          • McCoy’s 5A
          • Non-CO2 Media
          • Non-CO2 Medium with serum
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        • Cell Numbers
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          • Hemocytometer
        • Freezing Mammalian Cells
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      • Plants
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    • Incineration
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    • Monthly Maintenance
    • Orders
      • Chemical delivery address
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    • Peroxide testing
    • Repairs
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      • Routine checks 2022
    • Sterilization
      • Autoclave
      • Autoclave bags
    • Weekly Inspections
      • Biohazard Waste
      • Facility Inspection Check List
  • Recipes
    • Amino Acids
      • Adenine hemi-sulfate
      • Histidine HCl
      • Tryptophan
    • Antibiotics
      • Amphotericin
      • Ampicillin
      • Antibiotic/Antimycotic
      • carbenicillin
      • Chloramphenicol
      • Cipro
      • Doxycycline
      • Erythromycin
      • G418
      • Kanamycin
      • Streptomycin
      • Tetracycline
      • Trichostatin A
      • Triclosan
    • Antibodies
      • Primary Antibodies
        • 5-mC (rabbit)
        • Actin (mouse)
        • Actin (rabbit)
        • Alkaline phosphatase (mouse)
        • Alpha-actinin (mouse)
        • Cadherin (mouse)
        • Collagen II
        • Collagen IV
        • Connexin (rabbit)
        • Connexin (rat)
        • DNMT1 (rabbit)
        • Golgi (mouse)
        • H3 (1B1B2)
        • H3K27me3
        • Hec1 (mouse)
        • Histone H3 phospho S10
        • Integrin (rat)
        • Ki67
        • Lamin A/C
        • Lamin B1
        • Lamin B2
        • Laminin
        • Laminin (mouse)
        • Laminin (rabbit)
        • LAMP1 (lysosomes) (mouse)
        • LAP2 (mouse)
        • Mucin (mouse)
        • Myosin (mouse)
        • Myosin (rabbit)
        • Neurofilament (rabbit)
        • Nuclear pore complex
        • Phospho H3 (rabbit)
        • PKC (rabbit)
        • Synaptic vesicle (mouse)
        • Troponin (mouse)
        • Tubulin alpha (rat)
        • Tubulin gamma (mouse)
        • Vinculin (mouse)
        • ZO1 (Rabbit)
      • Secondary Antibodies
        • Goat anti-mouse 488
        • Goat anti-mouse IgM TRITC
        • Goat anti-mouse TRITC
        • Goat anti-rabbit 546
        • Goat anti-rabbit FITC
        • Goat anti-rabbit red
        • Goat anti-rat FITC
        • Goat anti-rat TRITC
    • Buffers
      • 10X HBS (dry)
      • 10X HBS (stocks)
      • 1X HBS (stocks)
      • 1X TAE
      • 50X TAE stock
      • Acid Buffer for Seawater
      • Citric acid buffer
      • Cnidocyte washing buffer
      • DEB
      • Diluent B
      • Edwards Extraction Buffer
      • HBS (Ca-)
      • HBS/Ca/Mg
      • HBSS/Ca/Mg
      • HEPES
      • HotSHOT genomic DNA prep
        • HotSHOT DNA extraction prep
          • Alkaline Lysis Reagent
        • Neutralization Buffer
      • PBS
      • PBS-Tw for Marine Bio
      • PBS-Tw-Azide
      • PBS-Tw-Azide-BSA
      • PBX (for fish)
      • PBX/BSA
      • Sodium acetate buffer
      • Sodium borate buffer
      • T10E1
      • T10E5
      • TAE
      • Taq dilution buffer
      • TBE 10X stock
      • TBSTw
    • Dilutions
    • Enzymes
      • Restriction Enzymes
      • RNase-Free DNase
      • Trypsin
    • Fixatives
      • Aceto-alcohol Fixative
      • Cnidocyte fixation solution
      • Fixing adherent cells
      • Formaldehyde fix
      • Formaldehyde fix
      • Methanol fix
      • Paraformaldehyde Fix
      • Paraformaldehyde from powder
      • Paraglut
      • PFA 4% in 1/3 SW
      • PFA 5% for fish
    • Gel Electrophoresis
      • Practice Loading Dye
    • Gels
      • EtBr gel
      • Gel Rigs
      • Sybr Safe gel
    • Hormones & Inhibitors, etc.
      • Angiotensin
      • ATP
      • By compound
        • Blebbistatin
        • BMS 833923
        • BP-A
        • BP-S
        • Bradykinin
        • Brefeldin A
        • Caffeine
        • Cannabinol
        • Centrinone
        • Con A coverslips
        • Con A dishes
        • Concanavalin A
        • cyclin inhibitor
        • DAPT
        • GSA 10
        • H-89
        • Haloperidol
        • Hydrocorisone
        • Importazole
        • Insulin-like growth factor-1 receptor inhibitor
        • Ionomycin
        • IWP2
        • Jasplakinolide
        • Latrunculin
        • Melanocyte Stimulating Hormone
        • ML-7
        • Monastrol
        • Monensin
        • oxidopamine
        • Poly-L-Lysine
        • Retinol
        • Sodium valproate
      • By target
      • cyclopamine
      • Cytochalasin D
      • IGF-1R I
      • Nerve Growth Factor
      • Nocodazole
      • Plant hormones
        • ABA
        • Cycloheximide
      • PMSF
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      • EGTA
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      • fibronectin
      • Fluo-4 AM
      • Fluorescein
      • HU (Hydroxyurea)
      • IPTG
      • LDL
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      • LiOAc-SDS
      • Lithium Acetate
      • Lithium chloride
      • Magnesium chloride
      • Magnesium sulfate
      • Manganese chloride
      • Manganese sulfate
      • MES
      • PIPES
      • Pluronic F-127
      • Potassium acetate
      • Potassium chloride
      • Potassium ferricyanide
      • Potassium ferrocyanide
      • Potassium hydroxide
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      • Potassium phosphate
      • Potassium phosphate dibasic
      • Potassium phosphate monobasic
      • SDS
      • Sodium acetate
      • Sodium alginate
      • Sodium azide
      • Sodium bicarbonate
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      • Sodium hydroxide
      • Sodium phosphate
      • Sodium Phosphate
      • Sodium phosphate dibasic
      • Sodium phosphate monobasic
      • Sodium sulfate
      • Spermidine
      • Tris
      • Tris Base
      • Tris-HCl
      • Triton X
      • Tween 10%
    • Sugars
      • Glucose
      • Lactose
      • Mannitol
      • Sucrose

    immunostaining RNAi S2

    Posted on October 1, 2025October 1, 2025 by Katherine Dorfman
    • 1 con-A coverslip in a dish per student + backups (repeat day: 2 per group + backup)
    • coverslip forceps
    • 4% PFA
    • PBS-Tw-Azide
    • Rabbit anti-Phospho H3
    • Rat anti-tubulin
    • goat anti rabbit red (Cy3)
    • goat anti rat 488
    • DAPI mounting medium
    • nail polish
    Bio383H_S2cell_Immunofluorescence_2024Download

    • Home
    • Courses
      • Biology Courses in the ISB
        • Bio 499CB (Loomis Cancer)
        • Bioimaging
          • 2024 Bioimaging (Stephens)
            • HeLa 9-3
            • IF for 10/22
            • IF for 9/24
          • 2025 Bioimaging (Stephens)
          • Reagents for 6.1
        • Gene & Genome
          • 2022 Maresca
          • 2024 Maresca
            • Transformation 2024 Gene & Genome
          • 2025 Maresca
            • 2025-09-03
            • 2025-09-08
            • 2025-09-10
            • 2025-09-15
            • 2025-09-17
            • 2025-09-22
            • 2025-09-24
            • ds RNA treatment
            • gel extraction
            • Gel materials for 383
            • immunostaining RNAi S2
            • t-7 Ribomax
          • Arabidopsis labs
            • Arabidopsis Anatomy
            • Reagents for 5.1
          • PCR 2024 G&GA
        • Genetics Lab
          • 2025 genetics schedule
            • 2025 genetics Week 2
          • Dog Coat Color
            • Dog DNA Extraction
            • Dog Genes Summary
              • ASIP
              • defB103
              • TyrP1
            • MC1R
          • mail dog kit
          • Yeast Adenine Synthesis
        • histology
          • Composite Images
          • Histology Supplies
          • Immunofluorescence for histology
          • Paraffin embedding
        • Marine Biology Bio 424 (Akiko Okusu)
          • 2024 Fall Marine Bio
          • bench baskets
          • Student kits
        • NAP lab
        • Perfect Plant 2024
          • 2024/02/07
          • 2024/02/14
          • 2024/02/21
          • 2024/02/28
          • 2024/03/27
        • QBoC
      • Outreach
        • 2015 Eureka
        • 2016 Bio Bootcamp
        • 2017 bio boot camp
        • 2018 Bio Boot Camp
        • 2022 Bio Boot Camp
          • Master mix
        • 2022 Eureka
          • Staining solution NucBlue + Mitotracker
        • 2023 Boot Camp
          • Zebrafish finclip genotyping
        • 2024 Bio Bootcamp
        • BioBootCamp
        • Chestnut Middle School 2015
        • DIY
          • Paper Activities
        • MassBio
        • Middle school 2016
        • Science Quest 2014
        • Summer Genetics
    • Electronics and Equipment
      • 2021 (June) Motic inventory
      • Classroom pipettes
      • Computers, servers, etc.
        • Computer admin
      • Dissecting Scopes
      • Equipment Lending
      • Fluorescence Microscopes
        • microscope pictures
          • Stage Micrometer images
        • Phase Rings
      • Jenway Genova Spectrophotometer
      • Major equipment
        • Biological Safety Cabinets
        • CO2 Manifold
        • Plant growth chambers
          • HOBO Data Logger
        • Plate Robot
      • Microscopes
        • Nikon E200
      • Motic Camera & Software
      • Optima Plate Reader
        • Programs
        • Script for overnight growth and fluorescence
      • Other digital cameras
      • Pipettes and tips
        • Eppendorf Repeater
        • Part numbers
        • Prep Room Pipettes
          • Rainin EDP 1000
      • Projectors
      • Thermocyclers
      • Zeiss scopes
    • File sharing
      • Handouts
        • Colorimetric Pipetting Exercise
        • CoolLED handout
        • Fluorescence handouts
        • HHMI Lab Manuals
        • Statistics
      • Labels
        • 0.5 mL microtube labels
        • 1.5 mL microtube labels
        • Brother P-touch 55
        • dH2O labels
        • Drawer labels
        • Slide Labels
        • Spot Labels
    • ISB Facilities and Resources
      • Door Access
      • Equipment
      • ISB Rooms
        • Conference rooms
        • Keys
        • Lab Schedules
        • Lab Wing
        • Old Room Schedules
          • 2011 Fall
          • 2012 Fall
          • 2016 Fall
          • 2020 Spring
          • 2022 Fall
          • 2024 Spring
          • 264 S2024
          • 360 S2024
          • 364 S2024
          • 368 S2024
      • Suppliers
        • Addgene Inc.
        • Airgas
        • Carolina Biological
        • Eppendorf
        • Fisher custom oligos
        • Fisher Scientific
        • Fisher Scientific-Clonetech
        • Krackler Scientific, Inc.
        • Qiagen
        • Rainin
        • Scilogex
        • Sigma-Aldrich
        • Thermo Fisher Scientific-Acros Organics
        • Thorlabs
    • Protocols
      • Dishwashing
        • Detergent for Dishwasher
      • Drierite Regeneration
      • Husbandry
        • Anemones
          • Exaptasia
          • F/2 Medium for algae (424)
            • Trace minerals for F/2
            • Vitamins for F/2
          • Nematostella
            • Growth chamber spawning program
          • Sea Water
          • Sea Water for 424
          • Symbiodinium
        • Brine Shrimp for Anemones
        • Culturing S-cells
        • E. coli
          • Bacterial media
            • Freezing bacteria
            • Freezing media
            • LB agar
              • LB agar granules
              • LB agar powder
              • LB broth + agar
            • LB antibiotic
            • LB Broth
              • LB broth from mix
              • LB from scratch
              • LB-Amp-Ara
              • LB-sugars
            • MacConkey + bile+ CV
            • MacConkey agar
            • Min med for GFP cells
            • minimal medium (glycerol)
            • Mueller Hinton agar
            • P-Glo Bacterial media
          • BL21(DE3)
        • Mammalian Cell Culture
          • Bulk Coverslip Staining
          • Cell Culture Media
            • DC5 (DMEM for canine cancer)
            • DMEM
            • F10-Ham’s
            • FBS aliquots
            • Freezing Media
            • McCoy’s 5A
            • Non-CO2 Media
            • Non-CO2 Medium with serum
            • serum-free
          • Cell Numbers
            • Cell Density Estimation
            • Hemocytometer
          • Freezing Mammalian Cells
          • nucleofection
            • Mirus
            • Selection of nucleofected cells
          • Tissue Culture Facility
        • Plants
          • Arabidopsis
          • Fast Plants
        • S2 Cells
        • Yeast
          • Yeast cultures
            • Yeast Media
              • 284 Yeast Plate Code
              • MV
              • MV + Ade
              • YEAD
              • YED
              • YEKAc
              • YEPAD
              • YEPAD Freezing
              • YPD
          • Yeast experiments for honors
          • Yeast pix
      • Incineration
      • Mini-prep
        • QIAmp DNA Mini Kit
        • Zymo miniprep classic
      • Monthly Maintenance
      • Orders
        • Chemical delivery address
        • Display Easels
        • Ethanol orders
        • Labcoats
        • Misc supplies
      • Peroxide testing
      • Repairs
      • Safety Inspections
        • Routine checks 2022
      • Sterilization
        • Autoclave
        • Autoclave bags
      • Weekly Inspections
        • Biohazard Waste
        • Facility Inspection Check List
    • Recipes
      • Amino Acids
        • Adenine hemi-sulfate
        • Histidine HCl
        • Tryptophan
      • Antibiotics
        • Amphotericin
        • Ampicillin
        • Antibiotic/Antimycotic
        • carbenicillin
        • Chloramphenicol
        • Cipro
        • Doxycycline
        • Erythromycin
        • G418
        • Kanamycin
        • Streptomycin
        • Tetracycline
        • Trichostatin A
        • Triclosan
      • Antibodies
        • Primary Antibodies
          • 5-mC (rabbit)
          • Actin (mouse)
          • Actin (rabbit)
          • Alkaline phosphatase (mouse)
          • Alpha-actinin (mouse)
          • Cadherin (mouse)
          • Collagen II
          • Collagen IV
          • Connexin (rabbit)
          • Connexin (rat)
          • DNMT1 (rabbit)
          • Golgi (mouse)
          • H3 (1B1B2)
          • H3K27me3
          • Hec1 (mouse)
          • Histone H3 phospho S10
          • Integrin (rat)
          • Ki67
          • Lamin A/C
          • Lamin B1
          • Lamin B2
          • Laminin
          • Laminin (mouse)
          • Laminin (rabbit)
          • LAMP1 (lysosomes) (mouse)
          • LAP2 (mouse)
          • Mucin (mouse)
          • Myosin (mouse)
          • Myosin (rabbit)
          • Neurofilament (rabbit)
          • Nuclear pore complex
          • Phospho H3 (rabbit)
          • PKC (rabbit)
          • Synaptic vesicle (mouse)
          • Troponin (mouse)
          • Tubulin alpha (rat)
          • Tubulin gamma (mouse)
          • Vinculin (mouse)
          • ZO1 (Rabbit)
        • Secondary Antibodies
          • Goat anti-mouse 488
          • Goat anti-mouse IgM TRITC
          • Goat anti-mouse TRITC
          • Goat anti-rabbit 546
          • Goat anti-rabbit FITC
          • Goat anti-rabbit red
          • Goat anti-rat FITC
          • Goat anti-rat TRITC
      • Buffers
        • 10X HBS (dry)
        • 10X HBS (stocks)
        • 1X HBS (stocks)
        • 1X TAE
        • 50X TAE stock
        • Acid Buffer for Seawater
        • Citric acid buffer
        • Cnidocyte washing buffer
        • DEB
        • Diluent B
        • Edwards Extraction Buffer
        • HBS (Ca-)
        • HBS/Ca/Mg
        • HBSS/Ca/Mg
        • HEPES
        • HotSHOT genomic DNA prep
          • HotSHOT DNA extraction prep
            • Alkaline Lysis Reagent
          • Neutralization Buffer
        • PBS
        • PBS-Tw for Marine Bio
        • PBS-Tw-Azide
        • PBS-Tw-Azide-BSA
        • PBX (for fish)
        • PBX/BSA
        • Sodium acetate buffer
        • Sodium borate buffer
        • T10E1
        • T10E5
        • TAE
        • Taq dilution buffer
        • TBE 10X stock
        • TBSTw
      • Dilutions
      • Enzymes
        • Restriction Enzymes
        • RNase-Free DNase
        • Trypsin
      • Fixatives
        • Aceto-alcohol Fixative
        • Cnidocyte fixation solution
        • Fixing adherent cells
        • Formaldehyde fix
        • Formaldehyde fix
        • Methanol fix
        • Paraformaldehyde Fix
        • Paraformaldehyde from powder
        • Paraglut
        • PFA 4% in 1/3 SW
        • PFA 5% for fish
      • Gel Electrophoresis
        • Practice Loading Dye
      • Gels
        • EtBr gel
        • Gel Rigs
        • Sybr Safe gel
      • Hormones & Inhibitors, etc.
        • Angiotensin
        • ATP
        • By compound
          • Blebbistatin
          • BMS 833923
          • BP-A
          • BP-S
          • Bradykinin
          • Brefeldin A
          • Caffeine
          • Cannabinol
          • Centrinone
          • Con A coverslips
          • Con A dishes
          • Concanavalin A
          • cyclin inhibitor
          • DAPT
          • GSA 10
          • H-89
          • Haloperidol
          • Hydrocorisone
          • Importazole
          • Insulin-like growth factor-1 receptor inhibitor
          • Ionomycin
          • IWP2
          • Jasplakinolide
          • Latrunculin
          • Melanocyte Stimulating Hormone
          • ML-7
          • Monastrol
          • Monensin
          • oxidopamine
          • Poly-L-Lysine
          • Retinol
          • Sodium valproate
        • By target
        • cyclopamine
        • Cytochalasin D
        • IGF-1R I
        • Nerve Growth Factor
        • Nocodazole
        • Plant hormones
          • ABA
          • Cycloheximide
        • PMSF
        • Purmorphamine
        • purmorphomine
        • STLC
        • Taxol
        • THC
        • Thyroid hormones
        • Transferrin
        • Valinomycin
        • Vasopressin
        • Vitamin D
        • β17-Estradiol
      • Mammalian Culture Media
        • Plant growth media
          • LPGM
          • MS medium
      • Primers
      • Stains
        • Acridine Orange
        • Alexa Fluor 568 Phalloidin
        • Click-iT® Plus EdU Imaging Kits
          • Buffer Additive
          • EdU
          • Reaction Buffer
        • DAPI Fluoromount
        • DAPI in glycerol for fish
        • DiOC6(3)
        • DNA gel stains
          • 3-color LD + SYBR Safe
          • EtBr
            • EtBr removalEtBr
          • Home-made LD
            • Xylene Cyanol
          • Safe-Green
          • Smart-glow
          • SYBR Safe
        • ER Tracker
        • FITC phalloidin
        • Lysotracker
        • Mitotracker
          • Mitotracker GREEN
          • Mitotracker RED
        • NBD Ceramide
        • Nuc Blue
        • Nucleus-RFP
        • Phalloidin
          • Red phalloidins
          • Rhodamine Phalloidin
          • Texas Red-X Phalloidin
        • Phloroglucinol
        • PS-Speck
        • Syto RNASelect
        • Tetramethylrhodamine α-Bungarotoxin
        • TMRE
        • Toluidine blue
      • Stock solutions
        • Adenine
        • Boric acid
        • Calcium chloride
        • Calcium nitrate
        • Chelated Iron
        • Cholesterol
        • Cobalt Chloride
        • DNA standards
        • DTT
        • EDTA
        • EGTA
        • Ferric citrate
        • Ferrous sulfate
        • fibronectin
        • Fluo-4 AM
        • Fluorescein
        • HU (Hydroxyurea)
        • IPTG
        • LDL
        • Leucine
        • LiOAc-SDS
        • Lithium Acetate
        • Lithium chloride
        • Magnesium chloride
        • Magnesium sulfate
        • Manganese chloride
        • Manganese sulfate
        • MES
        • PIPES
        • Pluronic F-127
        • Potassium acetate
        • Potassium chloride
        • Potassium ferricyanide
        • Potassium ferrocyanide
        • Potassium hydroxide
        • Potassium nitrate
        • Potassium phosphate
        • Potassium phosphate dibasic
        • Potassium phosphate monobasic
        • SDS
        • Sodium acetate
        • Sodium alginate
        • Sodium azide
        • Sodium bicarbonate
        • Sodium chloride
        • Sodium ferric EDTA
        • Sodium hydroxide
        • Sodium phosphate
        • Sodium Phosphate
        • Sodium phosphate dibasic
        • Sodium phosphate monobasic
        • Sodium sulfate
        • Spermidine
        • Tris
        • Tris Base
        • Tris-HCl
        • Triton X
        • Tween 10%
      • Sugars
        • Glucose
        • Lactose
        • Mannitol
        • Sucrose

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