UMass week	Dates	Lab Topic	Lab Activities	Deliverables
Week 1	9/1-9/5	NO LAB THIS WEEK	NONE	Syllabus quiz Intro survey UV mutagenesis quiz
Week 2	9/8-9/12	Yeast observation & picking mutants	Observing wild type and mutant yeast Completing complementation activity Pipetting practice Choosing and subculturing new mutants for characterization	Prelab quiz
Week 3	9/15-9/19	Observing subculture plates & scientific communication 1 DNA extraction	Observing subculture plates Assembling stripe plates for mating Dissecting a scientific introduction Outlining the introduction for your yeast manuscript DNA extraction from subcultures	Prelab quiz Lab notebook WRITE YOUR INTRODUCTION!
Week 4	9/22-9/26	PCR & complementation testing	PCR amplification of mutant DNA extracts Complementation testing of mutants	Prelab quiz Lab notebook Introduction due (1/group)
Week 5	9/29-10/3	Gel electrophoresis I, DNA sequencing I & complementation assay interpretation	Gel electrophoresis of week 4 PCRs Packaging successful PCRs for sequencing Interpreting complementation tests Replica plating of complementation tests	Prelab quiz Lab notebook
Week 6	10/6-10/10	Yeast bioinformatics	Bioinformatic analysis of mutant sequences Interpretation of replica plates	Prelab quiz Lab notebook
Week 7	10/13-10/17	NO LAB THIS WEEK	NONE	WRITE YOUR METHODS & RESULTS!
Week 8	10/20-10/24	Dog DNA Extraction & PCR Amplification	DNA extraction from dog swabs Nanodrop spectrophotometry PCR amplification of DNA extracts	Prelab quizLab notebook Revised intro, methods & results due (1/group) WRITE YOUR ABSTRACT & DISCUSSION (1/student)
Week 9	10/27-10/31	Gel electrophoresis II and DNA sequencing II	Gel electrophoresis of dog PCRs Package successful PCRs for sequencing	Prelab quiz Lab notebook Final yeast manuscript due (1/student)
Week 10	11/3-1//17 (MWTh ONLY)	RFLP & Dog bioinformatics I 2nd dog gel	Restriction digests RFLP analysis of TYRP1 Bioinformatic analysis of MC1R	Prelab quiz Lab notebook
Week 11	11/10-11/14	NO LAB THIS WEEK	NONE
Week 12	11/17-11/21 Tu ONLY	RFLP & Dog bioinformatics I 2nd dog gel	Restriction digests RFLP analysis of TYRP1 Bioinformatic analysis of MC1R	Prelab quiz Lab notebook
	11/17-11/21 MWTh ONLY	Dog bioinformatics II	Bioinformatic analysis of ASIP and BDef Final dog data analysis Work on presentations	Prelab quiz Lab notebook
Week 13	11/24-11/28 Tu ONLY	Dog bioinformatics II	Bioinformatic analysis of ASIP and BDef Final dog data analysis Work on presentations	Prelab quiz Lab notebook
	11/24-11/28 MWTh ONLY	NO LAB THIS WEEK	NONE	PREP YOUR PRESENTATIONS!
Week 14	12/1-12/5	Presentation symposium	Present the results of your dog genotyping project	Oral presentations
Week 15	5/5-5/9	NO LAB THIS WEEK	NONE	Presentation metacognition assignment Complete your university course evals for bonus points!
Finals week		Final exam	Final exam	Final exam