Look for Programs here
Desktop/This PC/Local Disc (C:)/Users/Public/Public Downloads/PS1_Protocols
or
On Pstar2, even though the files say Polar star 1):
Desktop/This PC/Local Disc (C:)/Users/Public/Public Downloads/polarstar1/PS1_Protocols
| Program | Layout | method | ex | em | vol |
|---|---|---|---|---|---|
| BUG OD 1 | A&B | Abs | 595 | . | 250 |
| BUG_FLUOR 2 | A:H | FL | 486 | 520 | . |
| BUG_OD_SHAKE 3 | A:H | ABS | 595 | . | 200 |
| FL-ABS-BY-2 4 | C1:2, D | ABS | 485 | . | 150 |
| REPEATABILITY 5 | A & B | ABS | 485 | . | 150 |
| SERIAL2&5_ABS 6 | E1-5, F:H | ABS | 485 | . | 150 |
| SERIAL2&5_FL 7 | E1-5, F:H | FL | 485 | 520 | . |
| REPEATAILITY_FL 8 | A:B | FL | 485 | 520 | . |
Script for overnight growth and fluorescence
- Lab 3.1 (2022) counting bacteria: std curve OD vs population density ↩︎
- Lab 4.2 (2022) lac operon shake cells, then read OD for overnight growth curves – part of script “Grow&Glow” ↩︎
- Lab 4.2 (2022) lac operon after BugOD Shake,read FL for overnight growth curves– part of script “Grow&Glow” ↩︎
- Lab 1.2 serial dilution & plate reader sensitivity ↩︎
- Lab 1.2 pipetting consistency (ABS) ↩︎
- Lab 1.3 serial dilution; absorbance of fluorescein; std curve ↩︎
- Lab 1.3 serial dilution; fluorescence of fluorescein; std curve ↩︎
- (Lab 1.2) pipetting consistency (FL) not used in 2022 ↩︎