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Better Labs and Prep Rooms

Doing stuff in the ISB

    2024 Spring

    Posted on December 5, 2024June 13, 2025 by Jennifer Ye

    Calendars

    264 S2024
    360 S2024
    364 S2024
    368 S2024

    • Home
    • Courses
      • Biology Courses in the ISB
        • Bio 499CB (Loomis Cancer)
        • Bioimaging
          • 2024 Bioimaging (Stephens)
            • HeLa 9-3
            • IF for 10/22
            • IF for 9/24
          • 2025 Bioimaging (Stephens)
          • Reagents for 6.1
        • Gene & Genome
          • 2022 Maresca
          • 2024 Maresca
            • Transformation Gene & Genome
          • 2025 Maresca
            • 2025-09-03
            • 2025-09-08
            • 2025-09-10
            • 2025-09-15
            • 2025-09-17
            • 2025-09-22
            • 2025-09-24
            • ds RNA treatment
            • gel extraction
            • Gel materials for 383
            • Gibson Assembly
            • IF Demo
            • immunostaining RNAi S2
            • t-7 Ribomax
          • Arabidopsis labs
            • Arabidopsis Anatomy
            • Reagents for 5.1
          • PCR 2024 G&GA
        • Genetics Lab
          • 2025 genetics schedule
            • 2025 genetics Week 2
          • Dog Coat Color
            • Dog DNA Extraction
            • Dog Genes Summary
              • ASIP
              • defB103
              • TyrP1
            • MC1R
          • mail dog kit
          • Yeast Adenine Synthesis
        • histology
          • Composite Images
          • Histology Supplies
          • Immunofluorescence for histology
          • Paraffin embedding
        • Marine Biology Bio 424 (Akiko Okusu)
          • 2024 Fall Marine Bio
          • bench baskets
          • Student kits
        • NAP lab
        • Perfect Plant 2024
          • 2024/02/07
          • 2024/02/14
          • 2024/02/21
          • 2024/02/28
          • 2024/03/27
        • QBoC
      • Outreach
        • 2015 Eureka
        • 2016 Bio Bootcamp
        • 2017 bio boot camp
        • 2018 Bio Boot Camp
        • 2022 Bio Boot Camp
          • Master mix
        • 2022 Eureka
          • Staining solution NucBlue + Mitotracker
        • 2023 Boot Camp
          • Zebrafish finclip genotyping
        • 2024 Bio Bootcamp
        • BioBootCamp
        • Chestnut Middle School 2015
        • DIY
          • Paper Activities
        • MassBio
        • Middle school 2016
        • Science Quest 2014
        • Summer Genetics
    • Electronics and Equipment
      • 2021 (June) Motic inventory
      • Classroom pipettes
      • Computers, servers, etc.
        • Computer admin
      • Dissecting Scopes
      • Equipment Lending
      • Fluorescence Microscopes
        • microscope pictures
          • Stage Micrometer images
        • Phase Rings
      • Jenway Genova Spectrophotometer
      • Major equipment
        • Biological Safety Cabinets
        • CO2 Manifold
        • Plant growth chambers
          • HOBO Data Logger
        • Plate Robot
      • Microscopes
        • Nikon E200
      • Motic Camera & Software
      • Optima Plate Reader
        • Programs
        • Script for overnight growth and fluorescence
      • Other digital cameras
      • Pipettes and tips
        • Eppendorf Repeater
        • Part numbers
        • Prep Room Pipettes
          • Rainin EDP 1000
      • Projectors
      • Thermocyclers
      • Zeiss scopes
    • File sharing
      • Handouts
        • Colorimetric Pipetting Exercise
        • Fluorescence handouts
          • CoolLED
          • Transmitted light
        • HHMI Lab Manuals
        • Statistics
        • Statistics
      • Labels
        • 0.5 mL microtube labels
        • 1.5 mL microtube labels
        • Brother P-touch 55
        • dH2O labels
        • Drawer labels
        • Slide Labels
        • Spot Labels
    • ISB Facilities and Resources
      • Door Access
      • Equipment
      • ISB Rooms
        • Conference rooms
        • Keys
        • Lab Schedules
        • Lab Wing
        • Old Room Schedules
          • 2011 Fall
          • 2012 Fall
          • 2016 Fall
          • 2020 Spring
          • 2022 Fall
          • 2024 Spring
          • 264 S2024
          • 360 S2024
          • 364 S2024
          • 368 S2024
      • Suppliers
        • Addgene Inc.
        • Airgas
        • Carolina Biological
        • Eppendorf
        • Fisher custom oligos
        • Fisher Scientific
        • Fisher Scientific-Clonetech
        • Krackler Scientific, Inc.
        • Qiagen
        • Rainin
        • Scilogex
        • Sigma-Aldrich
        • Thermo Fisher Scientific-Acros Organics
        • Thorlabs
    • Protocols
      • Dishwashing
        • Detergent for Dishwasher
      • Drierite Regeneration
      • Husbandry
        • Anemones
          • Exaptasia
          • F/2 Medium for algae (424)
            • Trace minerals for F/2
            • Vitamins for F/2
          • Nematostella
            • Growth chamber spawning program
          • Sea Water
          • Sea Water for 424
          • Symbiodinium or Breviolum
        • Brine Shrimp for Anemones
        • Culturing S-cells
        • E. coli
          • Bacterial media
            • Freezing bacteria
            • LB agar
              • LB agar granules
              • LB agar powder
              • LB broth + agar
            • LB antibiotic
            • LB Broth
              • LB broth from mix
              • LB from scratch
              • LB-Amp-Ara
              • LB-sugars
            • MacConkey + bile+ CV
            • MacConkey agar
            • Min med for GFP cells
            • minimal medium (glycerol)
            • Mueller Hinton agar
            • P-Glo Bacterial media
          • BL21(DE3)
        • Mammalian Cell Culture
          • Bulk Coverslip Staining
          • Cell Numbers
            • Cell Density Estimation
            • Hemocytometer
          • Freezing Mammalian Cells
          • nucleofection
            • Mirus
            • Selection of nucleofected cells
          • Plating
          • Tissue Culture Facility
        • Plants
          • Arabidopsis
          • Fast Plants
        • S2 Cells
        • Yeast
          • Yeast cultures
          • Yeast experiments for honors
          • Yeast pix
      • Incineration
      • Mini-prep
        • QIAmp DNA Mini Kit
        • Zymo miniprep classic
      • Monthly Maintenance
      • Orders
        • Chemical delivery address
        • Display Easels
        • Ethanol orders
        • Labcoats
        • Misc supplies
      • Peroxide testing
      • Repairs
      • Safety Inspections
        • Routine checks 2022
      • Sterilization
        • Autoclave
        • Autoclave bags
      • Weekly Inspections
        • Biohazard Waste
        • Facility Inspection Check List
    • Recipes
      • Amino Acids
        • Adenine hemi-sulfate
        • Histidine HCl
        • Tryptophan
      • Antibiotics
        • Amphotericin
        • Ampicillin
        • Antibiotic/Antimycotic
        • carbenicillin
        • Chloramphenicol
        • Cipro
        • Doxycycline
        • Erythromycin
        • G418
        • Kanamycin
        • Streptomycin
        • Tetracycline
        • Trichostatin A
        • Triclosan
      • Antibodies
        • Primary Antibodies
          • 5-mC (rabbit)
          • Actin (mouse)
          • Actin (rabbit)
          • Alkaline phosphatase (mouse)
          • Alpha-actinin (mouse)
          • Cadherin (mouse)
          • Collagen II
          • Collagen IV
          • Connexin (rabbit)
          • Connexin (rat)
          • DNMT1 (rabbit)
          • Golgi (mouse)
          • H3 (1B1B2)
          • H3K27me3
          • Hec1 (mouse)
          • Histone H3 phospho S10
          • Integrin (rat)
          • Ki67
          • Lamin A/C
          • Lamin B1
          • Lamin B2
          • Laminin
          • Laminin (mouse)
          • Laminin (rabbit)
          • LAMP1 (lysosomes) (mouse)
          • LAP2 (mouse)
          • Mucin (mouse)
          • Myosin (mouse)
          • Myosin (rabbit)
          • Neurofilament (rabbit)
          • Nuclear pore complex
          • Phospho H3 (rabbit)
          • PKC (rabbit)
          • Synaptic vesicle (mouse)
          • Troponin (mouse)
          • Tubulin alpha (rat)
          • Tubulin gamma (mouse)
          • Vinculin (mouse)
          • ZO1 (Rabbit)
        • Secondary Antibodies
          • Goat anti-mouse 488
          • goat anti-mouse 568
          • Goat anti-mouse IgM TRITC
          • Goat anti-mouse TRITC
          • Goat anti-rabbit 546
          • Goat anti-rabbit FITC
          • Goat anti-rabbit red
          • Goat anti-rat FITC
          • Goat anti-rat TRITC
      • Buffers
        • 1X TAE
        • 50X TAE stock
        • Acid Buffer for Seawater
        • Citric acid buffer
        • Cnidocyte washing buffer
        • DEB
        • Diluent B
        • Edwards Extraction Buffer
        • HBS
          • 10X HBS (dry)
          • 10X HBS (stocks)
          • 1X HBS (stocks)
          • HBS (Ca-)
          • HBS/Ca/Mg
        • HBSS/Ca/Mg
        • HEPES
        • HotSHOT genomic DNA prep
          • HotSHOT DNA extraction prep
            • Alkaline Lysis Reagent
          • Neutralization Buffer
        • PBS
          • PBS-Tw for Marine Bio
          • PBS-Tw-Azide
          • PBS-Tw-Azide-BSA
          • PBX (for fish)
          • PBX/BSA
        • Sodium acetate buffer
        • Sodium borate buffer
        • T10E1
        • T10E5
        • TAE
        • Taq dilution buffer
        • TBE 10X stock
        • TBSTw
      • Culture Media
        • L-15
        • Mammalian Culture Media
          • DC5 (DMEM for canine cancer)
          • DMEM
          • F10-Ham’s
          • FBS aliquots
          • Freezing media
          • McCoy’s 5A
          • Non-CO2 Medium with serum
            • Non-CO2 Media
          • serum-free
        • Plant growth media
          • LPGM
          • MS medium
        • Yeast Media
          • 284 Yeast Plate Code
          • MV
          • MV + Ade
          • YEAD
          • YED
          • YEKAc
          • YEPAD
          • YEPAD Freezing
          • YPD
      • Dilutions
        • DNA concentration
      • Enzymes
        • Restriction Enzymes
        • RNase-Free DNase
        • Trypsin
      • Fixatives
        • Aceto-alcohol Fixative
        • Cnidocyte fixation solution
        • Fixing adherent cells
        • Formaldehyde fix
        • Formaldehyde fix
        • Methanol fix
        • Paraformaldehyde Fix
        • Paraformaldehyde from powder
        • Paraglut
        • PFA 4% in 1/3 SW
        • PFA 5% for fish
      • Gel Electrophoresis
        • Practice Loading Dye
      • Gels
        • EtBr gel
        • Gel Rigs
        • Sybr Safe gel
      • Hormones & Inhibitors, etc.
        • Angiotensin
        • ATP
        • By compound
          • Blebbistatin
          • BMS 833923
          • BP-A
          • BP-S
          • Bradykinin
          • Brefeldin A
          • Caffeine
          • Cannabinol
          • Centrinone
          • Con A coverslips
          • Con A dishes
          • Concanavalin A
          • cyclin inhibitor
          • DAPT
          • GSA 10
          • H-89
          • Haloperidol
          • Hydrocorisone
          • Importazole
          • Insulin-like growth factor-1 receptor inhibitor
          • Ionomycin
          • IWP2
          • Jasplakinolide
          • Latrunculin
          • Melanocyte Stimulating Hormone
          • ML-7
          • Monastrol
          • Monensin
          • oxidopamine
          • Poly-L-Lysine
          • Retinol
          • Sodium valproate
        • By target
        • cyclopamine
        • Cytochalasin D
        • IGF-1R I
        • Nerve Growth Factor
        • Nocodazole
        • Plant hormones
          • ABA
          • Cycloheximide
        • PMSF
        • Purmorphamine
        • purmorphomine
        • STLC
        • Taxol
        • THC
        • Thyroid hormones
        • Transferrin
        • Valinomycin
        • Vasopressin
        • Vitamin D
        • β17-Estradiol
      • Primers
      • Stains
        • Acridine Orange
        • Alexa Fluor 568 Phalloidin
        • Click-iT® Plus EdU Imaging Kits
          • Buffer Additive
          • EdU
          • Reaction Buffer
        • DAPI Fluoromount
        • DAPI in glycerol for fish
        • DiOC6(3)
        • DNA gel stains
          • 3-color LD + SYBR Safe
          • EtBr
            • EtBr removalEtBr
          • Home-made LD
            • Xylene Cyanol
          • Safe-Green
          • Smart-glow
          • SYBR Safe
        • ER Tracker
        • FITC phalloidin
        • Lysotracker
        • Mitotracker
          • Mitotracker GREEN
          • Mitotracker RED
        • NBD Ceramide
        • Nuc Blue
        • Nucleus-RFP
        • Phalloidin
          • Red phalloidins
          • Rhodamine Phalloidin
          • Texas Red-X Phalloidin
        • Phloroglucinol
        • PS-Speck
        • Syto RNASelect
        • Tetramethylrhodamine α-Bungarotoxin
        • TMRE
        • Toluidine blue
      • Stock solutions
        • Adenine
        • Boric acid
        • Calcium chloride
        • Calcium nitrate
        • Chelated Iron
        • Cholesterol
        • Cobalt Chloride
        • DNA standards
        • DTT
        • EDTA
        • EGTA
        • Ferric citrate
        • Ferrous sulfate
        • fibronectin
        • Fluo-4 AM
        • Fluorescein
        • HU (Hydroxyurea)
        • IPTG
        • LDL
        • Leucine
        • LiOAc-SDS
        • Lithium Acetate
        • Lithium chloride
        • Magnesium chloride
        • Magnesium sulfate
        • Manganese chloride
        • Manganese sulfate
        • MES
        • PIPES
        • Pluronic F-127
        • Potassium acetate
        • Potassium chloride
        • Potassium ferricyanide
        • Potassium ferrocyanide
        • Potassium hydroxide
        • Potassium nitrate
        • Potassium phosphate
        • Potassium phosphate dibasic
        • Potassium phosphate monobasic
        • SDS
        • Sodium acetate
        • Sodium alginate
        • Sodium azide
        • Sodium bicarbonate
        • Sodium chloride
        • Sodium ferric EDTA
        • Sodium hydroxide
        • Sodium phosphate
        • Sodium Phosphate
        • Sodium phosphate dibasic
        • Sodium phosphate monobasic
        • Sodium sulfate
        • Spermidine
        • Tris
        • Tris Base
        • Tris-HCl
        • Triton X
        • Tween 10%
      • Sugars
        • Glucose
        • Lactose
        • Mannitol
        • Sucrose

     

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