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Better Labs and Prep Rooms
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Better Labs and Prep Rooms
Courses
Biology Courses in the ISB
Bio 499CB (Loomis Cancer)
Bioimaging
2024 Bioimaging (Stephens)
HeLa 9-3
IF for 10/22
IF for 9/24
2025 Bioimaging (Stephens)
Reagents for 6.1
Gene & Genome
2022 Maresca
2024 Maresca
Transformation 2024 Gene & Genome
2025 Maresca
Gel materials for 383
Arabidopsis labs
Arabidopsis Anatomy
Reagents for 5.1
PCR 2024 G&GA
Genetics Lab
2025 genetics schedule
2025 genetics Week 2
Dog Coat Color
Dog DNA Extraction
Dog Genes Summary
ASIP
defB103
TyrP1
MC1R
mail dog kit
Yeast Adenine Synthesis
histology
Composite Images
Histology Supplies
Immunofluorescence for histology
Paraffin embedding
Marine Biology Bio 424 (Akiko Okusu)
2024 Fall Marine Bio
bench baskets
Student kits
NAP lab
Perfect Plant 2024
2024/02/07
2024/02/14
2024/02/21
2024/02/28
2024/03/27
QBoC
Outreach
2015 Eureka
2016 Bio Bootcamp
2017 bio boot camp
2018 Bio Boot Camp
2022 Bio Boot Camp
Master mix
2022 Eureka
Staining solution NucBlue + Mitotracker
2023 Boot Camp
Zebrafish finclip genotyping
2024 Bio Bootcamp
BioBootCamp
Chestnut Middle School 2015
DIY
Paper Activities
MassBio
Middle school 2016
Science Quest 2014
Summer Genetics
Electronics and Equipment
2021 (June) Motic inventory
Classroom pipettes
Computers, servers, etc.
Computer admin
Dissecting Scopes
Equipment Lending
Fluorescence Microscopes
microscope pictures
Stage Micrometer images
Phase Rings
Jenway Genova Spectrophotometer
Major equipment
Biological Safety Cabinets
CO2 Manifold
Plant growth chambers
HOBO Data Logger
Plate Robot
Microscopes
Nikon E200
Motic Camera & Software
Optima Plate Reader
Programs
Script for overnight growth and fluorescence
Other digital cameras
Pipettes and tips
Eppendorf Repeater
Part numbers
Prep Room Pipettes
Rainin EDP 1000
Projectors
Thermocyclers
Zeiss scopes
File sharing
Handouts
Colorimetric Pipetting Exercise
CoolLED handout
Fluorescence handouts
HHMI Lab Manuals
Statistics
Labels
0.5 mL microtube labels
1.5 mL microtube labels
Brother P-touch 55
dH2O labels
Drawer labels
Slide Labels
Spot Labels
gel extraction
ISB Facilities and Resources
Door Access
Equipment
ISB Rooms
Conference rooms
Keys
Lab Schedules
Lab Wing
Old Room Schedules
2011 Fall
2012 Fall
2016 Fall
2020 Spring
2022 Fall
2024 Spring
264 S2024
360 S2024
364 S2024
368 S2024
Suppliers
Addgene Inc.
Airgas
Carolina Biological
Eppendorf
Fisher custom oligos
Fisher Scientific
Fisher Scientific-Clonetech
Krackler Scientific, Inc.
Qiagen
Rainin
Scilogex
Sigma-Aldrich
Thermo Fisher Scientific-Acros Organics
Thorlabs
Protocols
Dishwashing
Detergent for Dishwasher
Drierite Regeneration
Husbandry
Anemones
Exaptasia
F/2 Medium for algae (424)
Trace minerals for F/2
Vitamins for F/2
Nematostella
Growth chamber spawning program
Sea Water
Sea Water for 424
Symbiodinium
Brine Shrimp for Anemones
Culturing S-cells
E. coli
Bacterial media
Freezing bacteria
Freezing media
LB agar
LB agar granules
LB agar powder
LB broth + agar
LB antibiotic
LB Broth
LB broth from mix
LB from scratch
LB-Amp-Ara
LB-sugars
MacConkey + bile+ CV
MacConkey agar
Min med for GFP cells
minimal medium (glycerol)
Mueller Hinton agar
P-Glo Bacterial media
BL21(DE3)
Mammalian Cell Culture
Bulk Coverslip Staining
Cell Culture Media
DC5 (DMEM for canine cancer)
DMEM
F10-Ham’s
FBS aliquots
Freezing Media
McCoy’s 5A
Non-CO2 Media
Non-CO2 Medium with serum
serum-free
Cell Numbers
Cell Density Estimation
Hemocytometer
Freezing Mammalian Cells
nucleofection
Mirus
Selection of nucleofected cells
Tissue Culture Facility
Plants
Arabidopsis
Fast Plants
S2 Cells
Yeast
Yeast cultures
Yeast Media
284 Yeast Plate Code
MV
MV + Ade
YEAD
YED
YEKAc
YEPAD
YEPAD Freezing
YPD
Yeast experiments for honors
Yeast pix
Incineration
Mini-prep
QIAmp DNA Mini Kit
Zymo miniprep classic
Monthly Maintenance
Orders
Chemical delivery address
Display Easels
Ethanol orders
Labcoats
Misc supplies
Peroxide testing
Repairs
Safety Inspections
Routine checks 2022
Sterilization
Autoclave
Autoclave bags
Weekly Inspections
Biohazard Waste
Facility Inspection Check List
Recipes
Amino Acids
Adenine hemi-sulfate
Histidine HCl
Tryptophan
Antibiotics
Amphotericin
Ampicillin
Antibiotic/Antimycotic
carbenicillin
Chloramphenicol
Cipro
Doxycycline
Erythromycin
G418
Kanamycin
Streptomycin
Tetracycline
Trichostatin A
Triclosan
Antibodies
Primary Antibodies
5-mC (rabbit)
Actin (mouse)
Actin (rabbit)
Alkaline phosphatase (mouse)
Alpha-actinin (mouse)
Cadherin (mouse)
Collagen II
Collagen IV
Connexin (rabbit)
Connexin (rat)
DNMT1 (rabbit)
Golgi (mouse)
Hec1 (mouse)
Histone H3 phospho S10
Integrin (rat)
Ki67
Lamin A/C
Lamin B1
Lamin B2
Laminin
Laminin (mouse)
Laminin (rabbit)
LAMP1 (lysosomes) (mouse)
LAP2 (mouse)
Mucin (mouse)
Myosin (mouse)
Myosin (rabbit)
Neurofilament (rabbit)
Nuclear pore complex
Phospho H3 (rabbit)
PKC (rabbit)
Synaptic vesicle (mouse)
Troponin (mouse)
Tubulin alpha (rat)
Tubulin gamma (mouse)
Vinculin (mouse)
ZO1 (Rabbit)
Secondary Antibodies
Goat anti-mouse 488
Goat anti-mouse IgM TRITC
Goat anti-mouse TRITC
Goat anti-rabbit 546
Goat anti-rabbit FITC
Goat anti-rabbit red
Goat anti-rat FITC
Goat anti-rat TRITC
Buffers
10X HBS (dry)
10X HBS (stocks)
1X HBS (stocks)
1X TAE
50X TAE stock
Acid Buffer for Seawater
Citric acid buffer
Cnidocyte washing buffer
DEB
Diluent B
Edwards Extraction Buffer
HBS (Ca-)
HBS/Ca/Mg
HBSS/Ca/Mg
HEPES
HotSHOT genomic DNA prep
HotSHOT DNA extraction prep
Alkaline Lysis Reagent
Neutralization Buffer
PBS
PBS-Tw for Marine Bio
PBS-Tw-Azide
PBS-Tw-Azide-BSA
PBX (for fish)
PBX/BSA
Sodium acetate buffer
Sodium borate buffer
T10E1
T10E5
TAE
Taq dilution buffer
TBE 10X stock
TBSTw
Dilutions
Enzymes
Restriction Enzymes
RNase-Free DNase
Trypsin
Fixatives
Aceto-alcohol Fixative
Cnidocyte fixation solution
Fixing adherent cells
Formaldehyde fix
Formaldehyde fix
Methanol fix
Paraformaldehyde Fix
Paraformaldehyde from powder
Paraglut
PFA 4% in 1/3 SW
PFA 5% for fish
Gel Electrophoresis
Practice Loading Dye
Gels
EtBr gel
Gel Rigs
Sybr Safe gel
Hormones & Inhibitors, etc.
Angiotensin
ATP
By compound
Blebbistatin
BMS 833923
BP-A
BP-S
Bradykinin
Brefeldin A
Caffeine
Cannabinol
Centrinone
Concanavalin A
cyclin inhibitor
DAPT
GSA 10
H-89
Haloperidol
Hydrocorisone
Importazole
Insulin-like growth factor-1 receptor inhibitor
Ionomycin
IWP2
Jasplakinolide
Latrunculin
Melanocyte Stimulating Hormone
ML-7
Monastrol
Monensin
oxidopamine
Poly-L-Lysine
Retinol
Sodium valproate
By target
cyclopamine
Cytochalasin D
IGF-1R I
Nerve Growth Factor
Nocodazole
Plant hormones
ABA
Cycloheximide
PMSF
Purmorphamine
purmorphomine
STLC
Taxol
THC
Thyroid hormones
Transferrin
Valinomycin
Vasopressin
Vitamin D
β17-Estradiol
Mammalian Culture Media
Plant growth media
LPGM
MS medium
Primers
Stains
Acridine Orange
Alexa Fluor 568 Phalloidin
Click-iT® Plus EdU Imaging Kits
Buffer Additive
EdU
Reaction Buffer
DAPI Fluoromount
DAPI in glycerol for fish
DiOC6(3)
DNA gel stains
3-color LD + SYBR Safe
EtBr
EtBr removalEtBr
Home-made LD
Xylene Cyanol
Safe-Green
Smart-glow
SYBR Safe
ER Tracker
FITC phalloidin
Lysotracker
Mitotracker
Mitotracker GREEN
Mitotracker RED
NBD Ceramide
Nuc Blue
Nucleus-RFP
Phalloidin
Red phalloidins
Rhodamine Phalloidin
Texas Red-X Phalloidin
Phloroglucinol
PS-Speck
Syto RNASelect
Tetramethylrhodamine α-Bungarotoxin
TMRE
Toluidine blue
Stock solutions
Adenine
Boric acid
Calcium chloride
Calcium nitrate
Chelated Iron
Cholesterol
Cobalt Chloride
DNA standards
DTT
EDTA
EGTA
Ferric citrate
Ferrous sulfate
fibronectin
Fluo-4 AM
Fluorescein
HU (Hydroxyurea)
IPTG
LDL
Leucine
LiOAc-SDS
Lithium Acetate
Lithium chloride
Magnesium chloride
Magnesium sulfate
Manganese chloride
Manganese sulfate
MES
PIPES
Pluronic F-127
Potassium acetate
Potassium chloride
Potassium ferricyanide
Potassium ferrocyanide
Potassium hydroxide
Potassium nitrate
Potassium phosphate
Potassium phosphate dibasic
Potassium phosphate monobasic
SDS
Sodium acetate
Sodium alginate
Sodium azide
Sodium bicarbonate
Sodium chloride
Sodium ferric EDTA
Sodium hydroxide
Sodium phosphate
Sodium Phosphate
Sodium phosphate dibasic
Sodium phosphate monobasic
Sodium sulfate
Spermidine
Tris
Tris Base
Tris-HCl
Triton X
Tween 10%
Sugars
Glucose
Lactose
Mannitol
Sucrose
Con A coverslips
Con A dishes
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Cell Culture Media
Doing stuff in the ISB