5 liters of f/2 medium for algae.

L1 Medium Kit from Bigelow NCMA contains the nitrate, phosphate, trace minerals, and vitamins. Add the antibiotics from prep room freezer stocks.

Re-inoculate once during week of 1/15

TA & Students will re-inoculate once monthly

50 mL/month x 4 months x 16 students = 3200 mL

Make 4 L for the semester.

To make (in a sterile hood)	L F/2 medium for algae
add:	mL NaNO3 (75g/L)
add:	mL NaHPO4 (5 g/L)
add:	mL Trace minerals
add:	mL vitamins
add:	mL kanamycin
add:	mL ampicillin
add:	mL streptomycin

Plus ASW to final volume

Filter sterilize

To care for algae:

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• Clean flasks with 1M HCl, then rinse multiple times, finishing with RO water
• Autoclave with silicone sponge closures
• Aliquot 50 mL F/2 medium into sterile flasks
• Seed with 1 mL of month old algal culture
• Incubate in growth chamber