For motility strains
| To make | mL min med |
| start with | mL 10X M9 |
| add: | mL 200X HMLTT |
| add: | mL 100% glycerol |
| add: | mL H2O |
| add: | mL 1M MgSO4 |
| add: | uL 1M CaCl2, stir if it precipitates |
Bring to final volume
For motility strains
| To make | mL min med |
| start with | mL 10X M9 |
| add: | mL 200X HMLTT |
| add: | mL 100% glycerol |
| add: | mL H2O |
| add: | mL 1M MgSO4 |
| add: | uL 1M CaCl2, stir if it precipitates |
Bring to final volume