Buy from Fisher TAE 50x, pH 8.3, 4L, part #BP13324
from Maniotis
pH to 8 with acetic acid or NaOH
should this be 8.4?
| Ingredient | cf (mM) | 1000 | 750 | 600 | 500 | mL |
|---|---|---|---|---|---|---|
| Tris | 2000 | 242 | 181.5 | 145.2 | 121 | g |
| Acetic acid | 1000 | 57.1 | 42.9 | 34.3 | 28.6 | mL |
| EDTA 0.5 M | 50 | 100 | 75 | 60 | 50 | mL |
| OR | ||||||
| EDTA | 50 | 18.62 | 13.965 | 11.172 | 9.31 | g |
pH to 8 with acetic acid or NaOH
Maniotis says to autoclave, but the salt concentration is so high that nothing will grow in it if you don’t.