To dilute Takara Ex-Taq

Store in freezer. Won’t freeze solid. Should keep forever.

• 50 μL Tween 20
• 50 μL Nonidet P-40 (anionic detergent = NP-40)
• 10 µL 1M DTT
• 1 mL KCl (1M)
• 200 μL Tris-Cl pH 8 (1M)
• 2 μL 0.5M EDTA pH 8
• 5 mL glycerol
• 4.2 mL ddH2O

To make	mL Taq dilution buffer
add:	mL Tris 1M pH 8
add:	mL KCl 1M
add:	mL EDTA 0.5M
add:	mL DTT 1M
add:	mL Tween 20
add	mL Nonidet P-40
add	mL glycerol

bring to final volume, filter sterilize in BSC, freeze

Ex-Taq user manual