Sporulation Medium for Bio 284

Yeast Extract Potassium Acetate

If you have concentrated potassium acetate solution, then:

• 20.4 mL 5M KOAc per liter YEKAc
• 12.75 mL 8M KOAc per liter YEKAc Fisher AAJ63372AE

To make	YEKAC agar plates
at	mm plate diameter
at	KOAc Molarity
pour	mL per plate
for a final volume of:	mL water
start with:	mL water
add:	mL KOAc, stir till dissolved
add:	g yeast extract, stir till dissolved
add:	g agar, leave stir bar in
autoclave for:	minutes

If you are using potassium acetate powder, then:

To make	YEKAC agar plates
at	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g potassium acetate, stir till dissolved
add:	g yeast extract, stir till dissolved
add:	g agar, leave stir bar in
autoclave for:	minutes

Mark with a single black line.

Seal with green tape