Sybr Safe gel

1 µL SYBR Safe / 10 mL gel

Invitrogen S33102 – cheaper at Fisher than Thermo

50 mL gels:
%:
1X TAE: mL
agarose: g
SYBR: µL

*Add weighed agarose to measured TAE in a flask (about half the maximum volume of the flask)

*Heat in the microwave at full power until it just barely starts to boil.

*Boil in the microwave CAREFULLY (power level 0.1) until completely dissolved (check by swirling) DO NOT LET IT BOIL OVER

*Allow to cool slightly

*Add SYBR-safe

*Aliquot into 50 mL conical tubes

*Put in rack in 65C waterbath

Clean-up

Everything can go in the trash or down the drain