Requirements: Acid washed coverslips stored in 100% ethanol.1
- ConA 0.5 mg/mL.
- Large petri dishes to store the coverslips.
- Use tall petri dishes to culture cells: Fisher S24557 or Go Science Crazy 1500710
Procedure 1:
- Flame the acid-washed coverslips and cool for 10 sec.
(Make 9-14 coverslips at a time to save time.) - Place the coverslips on top of the large petri dish.
- Thaw the conA from the freezer and dilute to 0.5 mg/mL
- apply around 400uL to the first two coverslips
- Wait for 10 sec.
- Tilt the coverslips and suck out the excess ConA
- Repeat the same for all the coverslips.
- Cover the petri dish with another lid of petri dish and allow a small opening to dry.
- If possible,use a vacuum pump to dry it faster.
The process usually takes 15 minutes and drying takes half an hour.
Procedure 2:
- Put sterilized coverslips in holder, every other slot
- Dunk in ethanol, let dry overnight
- Dunk in 0.5 mg/mL concanavalin A solution, let dry
- Put in individual 15 mm x 35 mm sterile Petri dishes.
Storage:
Cover with the lid. 10 min before needed, UV sterilize them.
- Alternatively, UV sterilize all of them after drying and open the dishes only in aseptic conditions or inside hood.
- In a petri dish, place the coverslips on top of a clean kimwipe with the conA surface facing up. ↩︎