Concanavalin-coated coverslip dishes

• Start with 5 mg/mL ConA stock solution
• Keep sterile – work in sterile hood
• aliquot 100 µL into microfuge tubes  (cannot take repeated freeze-thaw cycles)
• Coat coverslip bottom dishes with either:
  • 0.75 mL 0.1 mg/mL:  0.015 mL (5 mg/mL) + 0.735 mL PBS
  • 0.75 mL 0.5 mg/mL:  0.075 mL (5 mg/mL) + 0.675 mL PBS
• Incubate the coverslip dishes for 1 hour 37˚C 
• Remove the solution
• rinse in PBS 
• Air dry the dish.