MW = 507.62
Sigma C2618: 200 µL 5 mg/mL (~10 mM) in DMSO
Sigma C8273: 5 mg. Dissolve in 1 mL DMSO
- alkaloid mycotoxin produced by Helminthosporium and other molds.
- disrupts actin microfilaments
- binds to F-actin polymer
- prevents polymerization of actin monomers
- Aliquot 10 µL 10 mM
- Add 90 uL DMSO (makes 1 mM)
- Dilute this 1:1000 into medium (makes 1uM)
- Dilute the 1 uM to desired working concentration
- Store in the dark, in the freezer
- Adding 490 µL of non-CO2 medium makes 500 µL of 200 µM
- Final dilution should contain no more than 0.1% DMSO
- 250 nM in the dish should give an effect
- 15 – 30 min