Materials required but not in kit:
- PBS
- 3.7% formaldehyde in PBS
- 0.5 % Triton-X in PBS
- 3% BSA in PBSTX
Stock Solutions
| Component | conc | dilute with |
|---|---|---|
| A (EdU) | 10 mM | 2 mL DMSO (C) or buffer |
| B (Alexa Fluor® picolyl azide) | ||
| C (DMSO) | ||
| D (reaction buffer) | 10X | |
| E Cu protectant | ||
| F (buffer additive) | ||
| G (Hoechst® 33342) |
Per tube, need 200uL