| ingredient | working conc | MW | stock |
|---|---|---|---|
| HU | 0.2 M | 76 | 2M in H2O |
Hydroxyurea: (Sigma H8267-1g)
from the Koshand lab
To arrest yeast
- To make 50 mL YPD with 0.2M HU
- vi = (0.2M * 50 mL)/2M = 5 mL
- 100µL 2M stock per mL medium
- Buy 1 g, make 6.6 mL 2M stock
- MW = 76, so 1 g = 1/76 mol = 0.0132 mol
- vol = (1000 mL/2 mol) * 0.0132 mol = 6.6 mL
See yeast recipes for QBoC