Slc30a9

Slc30a9 ^-/- embryos are not delayed at E7.5.

Slc30a9 ^-/- embryos at E8.5 are always smaller than their control littermates. Although only a minority of Slc30a9 ^-/- are categorized as delayed at E8.5 most display an abnormal allantois that is often blebby at the tip and irregularly shaped (see others below). The head shape is sometimes abnormal. By E9.5 all Slc30a9 ^-/- embryos are delayed, all have an abnormal head shape and abnormal yolk sacs, most have failed to close their cranial neural tube and all are unturned. In the majority of E9.5 Slc30a9 ^-/- embryos, instead of forming a functional connection with the chorion (as in the example provided), the allantois has balled up at the tip of the tail.

At E8.5, Slc30a9^-/- all placentas examined appear properly developed and comparable to littermate controls.

At E9.5, Slc30a9^-/- placentas exhibit impaired chorioallantoic attachment as well as multiple labyrinth zone defects when compared with controls. Failure of chorioallantoic attachment is observed in most KO conceptuses examined (n = 8/14). However, in the conceptuses with normal attachment (n = 6/14), less allantoic mesoderm is observed attached to the chorion compared to controls. In the KO placentas, the Tfeb+ region fails to expand, remaining a thin layer, and Gcm1+ chorioallantoic branching is severely impaired.

The E8.5 Slc30a9 ^-/- embryos have all 3 germ layers and appear appropriately patterned.

Section in situ hybridization of Slc30a9 in WT placentas (purple) reveals expression in the fetal compartment of the placenta (junctional and labyrinth zone). These sections are counterstained with eosin (pink).

Accession number: NM_178651.4