Publications

051. Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages.

Flores-Valdez MA, Peterson EJR, Aceves-Sánchez MJ, Baliga NS, Morita YS, Sparks IL, Saini DK, Yadav R, Lang R, Mata-Espinosa D, León-Contreras JC, Hernández-Pando R.

Sci Rep. 2024 May 24;14(1):11898. doi: 10.1038/s41598-024-61815-8. 

050. Detergent-induced quantitatively limited formation of diacyl phosphatidylinositol dimannoside in Mycobacterium smegmatis.

Kitzmiller CE, Cheng TY, Prandi J, Sparks IL, Moody DB, Morita YS.
 
J Lipid Res. 2024 Mar 22:100533. doi: 10.1016/j.jlr.2024.100533.
 
048. A cell wall synthase accelerates plasma membrane partitioning in mycobacteria.
 
Kado T, Akbary Z, Motooka D, Sparks IL, Melzer ES, Nakamura S, Rojas ER, Morita YS, Siegrist MS.
 
eLife. 2023 Sep 4;12:e81924. doi: 10.7554/eLife.81924. 
 
047. Tuberculostearic acid controls mycobacterial membrane compartmentalization.
 
Prithviraj M, Kado T, Mayfield JA, Young DC, Huang AD, Motooka D, Nakamura S, Siegrist MS, Moody DB, Morita YS.
 
mBio. 2023 Apr 25;14(2):e0339622. doi: 10.1128/mbio.03396-22.
 
044. An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria.
 
Gupta KR, Gwin CM, Rahlwes KC, Biegas KJ, Wang C, Park JH, Liu J, Swarts BM, Morita YS, Rego EH.
 
eLife. 2022 Nov 8;11:e80395. doi: 10.7554/eLife.80395.
 

043. Inositol acylation of phosphatidylinositol mannosides: a rapid mass response to membrane fluidization in mycobacteria.

Nguyen PP, Kado T, Prithviraj M, Siegrist MS, Morita YS.
 
J Lipid Res. 2022 Sep;63(9):100262. doi: 10.1016/j.jlr.2022.100262.
 

042. Cell wall damage reveals spatial flexibility in peptidoglycan synthesis and a nonredundant role for RodA in mycobacteria.

Melzer ES, Kado T, García-Heredia A, Gupta KR, Meniche X, Morita YS, Sassetti CM, Rego EH, Siegrist MS.

J Bacteriol. 2022 May 11:e0054021. doi: 10.1128/jb.00540-21.

041. Fluorescence imaging-based discovery of membrane domain-associated proteins in Mycobacterium smegmatis.

Rokicki CAZ, Brenner JR, Dills AH, Judd JJ, Kester JC, Puffal J, Sparks IL, Prithviraj M, Anderson BR, Wade JT, Gray TA, Derbyshire KM, Fortune SM, Morita YS.

J Bacteriol. 2021 Oct 25;203(22):e0041921. doi: 10.1128/JB.00419-21

040. Membrane-partitioned cell wall synthesis in mycobacteria.

García-Heredia A, Kado T, Sein CE, Puffal J, Osman SH, Judd J, Gray TA, Morita YS, Siegrist MS.

eLife. 2021 Feb 5;10:e60263. doi: 10.7554/eLife.60263.

Rahlwes KC, Osman SH, Morita YS.

mSphere. 2020 Nov 4;5(6):e01039-20. doi: 10.1128/mSphere.01039-20.

038. Photoactivatable glycolipid probes for identifying mycolate-protein interactions in live mycobacteria.

Kavunja HW, Biegas KJ, Banahene N, Stewart JA, Piligian BF, Groenevelt JM, Sein CE, Morita YS, Niederweis M, Siegrist MS, Swarts BM.

J Am Chem Soc. 2020 Apr 29;142(17):7725-7731. doi: 10.1021/jacs.0c01065.

037. Mycobacterial membrane domain, or a primordial organelle?

Hayashi JM, Morita YS.

Yale J Biol Med. 2019 Sep 20;92(3):549-556. eCollection 2019 Sep. Review.

036. Cell walls and membranes of actinobacteria.

Rahlwes KC, Sparks IL, Morita YS.

Subcell Biochem. 2019;92:417-469. doi: 10.1007/978-3-030-18768-2_13. Review.

035. Purification and analysis of mycobacterial phosphatidylinositol mannosides, lipomannan, and lipoarabinomannan.

Rahlwes KC, Puffal J, Morita YS.

Methods Mol Biol. 2019;1954:59-75. doi: 10.1007/978-1-4939-9154-9_6.

034. Demethylmenaquinone methyl transferase Is a membrane domain-associated protein essential for menaquinone homeostasis in Mycobacterium smegmatis.

Puffal J, Mayfield JA, Moody DB, Morita YS.

Front Microbiol. 2018 Dec 18;9:3145. doi: 10.3389/fmicb.2018.03145.

033. Sidewall cell envelope synthesis and remodeling in pole-growing mycobacteria.

García-Heredia A, Pohane AA, Melzer ES, Fiolek TJ, Rundell SR, Lim HC, Wagner J, Morita YS, Swarts BM, Siegrist MS.

eLife. 2018 Sep 10;7. pii: e37243. doi: 10.7554/eLife.37243.

032. Spatial control of cell envelope biosynthesis in mycobacteria.

Puffal J, García-Heredia A, Rahlwes KC, Siegrist MS, Morita YS.

Pathog Dis. 2018 Jun 1;76(4). doi: 10.1093/femspd/fty027. Review.

031. Deletion of PimE mannosyltransferase results in increased copper sensitivity in Mycobacterium smegmatis.

Eagen WJ, Baumoel LR, Osman SH, Rahlwes KC, Morita YS.

FEMS Microbiol Lett. 2018 Mar 1;365(6). doi: 10.1093/femsle/fny025.

030. Stress-induced reorganization of the mycobacterial membrane domain.

Hayashi JM, Richardson K, Melzer ES, Sandler SJ, Aldridge BB, Siegrist MS, Morita YS.

mBio. 2018 Jan 23;9(1). pii: e01823-17. doi: 10.1128/mBio.01823-17.

029. Characterization of conserved and novel septal factors in Mycobacterium smegmatis.

Wu KJ, Zhang J, Baranowski C, Leung V, Rego EH, Morita YS, Rubin EJ, Boutte CC.

J Bacteriol. 2018 Feb 23;200(6). pii: e00649-17. doi: 10.1128/JB.00649-17.

028. The cell envelope-associated phospholipid-binding protein LmeA is required for mannan polymerization in mycobacteria.

Rahlwes KC, Ha SA, Motooka D, Mayfield JA, Baumoel LR, Strickland JN, Torres-Ocampo AP, Nakamura S, Morita YS.

J Biol Chem. 2017 Oct 20;292(42):17407-17417. doi: 10.1074/jbc.M117.804377.

027. C-type lectin receptor DCAR recognizes mycobacterial phosphatidyl-inositol mannosides to promote a Th1 response during infection.

Toyonaga K, Torigoe S, Motomura Y, Kamichi T, Hayashi JM, Morita YS, Noguchi N, Chuma Y, Kiyohara H, Matsuo K, Tanaka H, Nakagawa Y, Sakuma T, Ohmuraya M, Yamamoto T, Umemura M, Matsuzaki G, Yoshikai Y, Yano I, Miyamoto T, Yamasaki S.

Immunity. 2016 Dec 20;45(6):1245-1257. doi: 10.1016/j.immuni.2016.10.012.

026. Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils.

Nakayama H, Kurihara H, Morita YS, Kinoshita T, Mauri L, Prinetti A, Sonnino S, Yokoyama N, Ogawa H, Takamori K, Iwabuchi K.

Sci Signal. 2016 Oct 11;9(449):ra101.

025. Spatially distinct and metabolically active membrane domain in mycobacteria.

Hayashi JM, Luo CY, Mayfield JA, Hsu T, Fukuda T, Walfield AL, Giffen SR, Leszyk JD, Baer CE, Bennion OT, Madduri A, Shaffer SA, Aldridge BB, Sassetti CM, Sandler SJ, Kinoshita T, Moody DB, Morita YS.

Proc Natl Acad Sci U S A. 2016 May 10;113(19):5400-5. doi: 10.1073/pnas.1525165113.

024. New insights into the functions of PIGF, a protein involved in the ethanolamine phosphate transfer steps of glycosylphosphatidylinositol biosynthesis.

Stokes MJ, Murakami Y, Maeda Y, Kinoshita T, Morita YS.

Biochem J. 2014 Oct 15;463(2):249-56. doi: 10.1042/BJ20140541.

023. Critical roles for lipomannan and lipoarabinomannan in cell wall integrity of mycobacteria and pathogenesis of tuberculosis.

Fukuda T, Matsumura T, Ato M, Hamasaki M, Nishiuchi Y, Murakami Y, Maeda Y, Yoshimori T, Matsumoto S, Kobayashi K, Kinoshita T, Morita YS.

mBio. 2013 Feb 19;4(1):e00472-12. doi: 10.1128/mBio.00472-12.

022. Identification of a second catalytically active trans-sialidase in Trypanosoma brucei.

Nakatani F, Morita YS, Ashida H, Nagamune K, Maeda Y, Kinoshita T.

Biochem Biophys Res Commun. 2011 Nov 18;415(2):421-5. doi: 10.1016/j.bbrc.2011.10.085. Epub 2011 Oct 21.

021. Inositol lipid metabolism in mycobacteria: biosynthesis and regulatory mechanisms.

Morita YS, Fukuda T, Sena CB, Yamaryo-Botte Y, McConville MJ, Kinoshita T.

Biochim Biophys Acta. 2011 Jun;1810(6):630-41. doi: 10.1016/j.bbagen.2011.03.017. Review.

020. Stress-induced synthesis of phosphatidylinositol 3-phosphate in mycobacteria.

Morita YS, Yamaryo-Botte Y, Miyanagi K, Callaghan JM, Patterson JH, Crellin PK, Coppel RL, Billman-Jacobe H, Kinoshita T, McConville MJ.

J Biol Chem. 2010 May 28;285(22):16643-50. doi: 10.1074/jbc.M110.119263.

019. Controlled expression of branch-forming mannosyltransferase is critical for mycobacterial lipoarabinomannan biosynthesis.

Sena CB, Fukuda T, Miyanagi K, Matsumoto S, Kobayashi K, Murakami Y, Maeda Y, Kinoshita T, Morita YS.

J Biol Chem. 2010 Apr 30;285(18):13326-36. doi: 10.1074/jbc.M109.077297.

018. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle.

Ishikawa E, Ishikawa T, Morita YS, Toyonaga K, Yamada H, Takeuchi O, Kinoshita T, Akira S, Yoshikai Y, Yamasaki S.

J Exp Med. 2009 Dec 21;206(13):2879-88. doi: 10.1084/jem.20091750.

017. Mutations in pimE restore lipoarabinomannan synthesis and growth in a Mycobacterium smegmatis lpqW mutant.

Crellin PK, Kovacevic S, Martin KL, Brammananth R, Morita YS, Billman-Jacobe H, McConville MJ, Coppel RL.

J Bacteriol. 2008 May;190(10):3690-9. doi: 10.1128/JB.00200-08.

016. PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria.

Morita YS, Sena CB, Waller RF, Kurokawa K, Sernee MF, Nakatani F, Haites RE, Billman-Jacobe H, McConville MJ, Maeda Y, Kinoshita T.

J Biol Chem. 2006 Sep 1;281(35):25143-55. doi: 10.1074/jbc.M604214200.

015. Removal or maintenance of inositol-linked acyl chain in glycosylphosphatidylinositol is critical in trypanosome life cycle.

Hong Y, Nagamune K, Morita YS, Nakatani F, Ashida H, Maeda Y, Kinoshita T.

J Biol Chem. 2006 Apr 28;281(17):11595-602. doi: 10.1074/jbc.M513061200.

014. Identification of a novel protein with a role in lipoarabinomannan biosynthesis in mycobacteria.

Kovacevic S, Anderson D, Morita YS, Patterson J, Haites R, McMillan BN, Coppel R, McConville MJ, Billman-Jacobe H.

J Biol Chem. 2006 Apr 7;281(14):9011-7. doi: 10.1074/jbc.M511709200.

013. TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei.

Hong Y, Nagamune K, Ohishi K, Morita YS, Ashida H, Maeda Y, Kinoshita T.

FEBS Lett. 2006 Jan 23;580(2):603-6. doi: 10.1016/j.febslet.2005.12.075.

012. Compartmentalization of lipid biosynthesis in mycobacteria.

Morita YS, Velasquez R, Taig E, Waller RF, Patterson JH, Tull D, Williams SJ, Billman-Jacobe H, McConville MJ.

J Biol Chem. 2005 Jun 3;280(22):21645-52. doi: 10.1074/jbc.M414181200.

011. Function of phosphatidylinositol in mycobacteria.

Haites RE, Morita YS, McConville MJ, Billman-Jacobe H.

J Biol Chem. 2005 Mar 25;280(12):10981-7. doi: 10.1074/jbc.M413443200.

010. PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol.

Kang JY, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita YS, Maeda Y, Kinoshita T.

J Biol Chem. 2005 Mar 11;280(10):9489-97. doi: 10.1074/jbc.M413867200.

009. Biosynthesis of mycobacterial phosphatidylinositol mannosides.

Morita YS, Patterson JH, Billman-Jacobe H, McConville MJ.

Biochem J. 2004 Mar 1;378(Pt 2):589-97. doi: 10.1042/BJ20031372.

008. Fatty acid synthesis in African trypanosomes: a solution to the myristate mystery.

Paul KS, Jiang D, Morita YS, Englund PT.

Trends Parasitol. 2001 Aug;17(8):381-7. doi: 10.1016/s1471-4922(01)01984-5. Review.

007. Fatty acid remodeling of glycosyl phosphatidylinositol anchors in Trypanosoma brucei: incorporation of fatty acids other than myristate.

Morita YS, Englund PT.

Mol Biochem Parasitol. 2001 Jul;115(2):157-64. doi: 10.1016/s0166-6851(01)00279-1.

006. Virulence of Trypanosoma brucei strain 427 is not affected by the absence of glycosylphosphatidylinositol phospholipase C.

Leal S, Acosta-Serrano A, Morita YS, Englund PT, Böhme U, Cross GA.

Mol Biochem Parasitol. 2001 May;114(2):245-7. doi: 10.1016/s0166-6851(01)00257-2.

005. Specialized fatty acid synthesis in African trypanosomes: myristate for GPI anchors.

Morita YS, Paul KS, Englund PT.

Science. 2000 Apr 7;288(5463):140-3. doi: 10.1126/science.288.5463.140.

004. Glycosyl phosphatidylinositol myristoylation in African trypanosomes. New intermediates in the pathway for fatty acid remodeling.

Morita YS, Acosta-Serrano A, Buxbaum LU, Englund PT.

J Biol Chem. 2000 May 12;275(19):14147-54. doi: 10.1074/jbc.275.19.14147.

 

003. Identification and characterization of protozoan products that trigger the synthesis of IL-12 by inflammatory macrophages.

Gazzinelli RT, Camargo MM, Almeida IC, Morita YS, Giraldo M, Acosta-Serrano A, Hieny S, Englund PT, Ferguson MA, Travassos LR, Sher A.

Chem Immunol. 1997;68:136-52. doi: 10.1159/000058689. Review.

 

002. Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements.

Günzl A, Ullu E, Dörner M, Fragoso SP, Hoffmann KF, Milner JD, Morita Y, Nguu EK, Vanacova S, Wünsch S, Dare AO, Kwon H, Tschudi C.

Mol Biochem Parasitol. 1997 Mar;85(1):67-76.

001. Localization of Dictyostelium myoB and myoD to filopodia and cell-cell contact sites using isoform-specific antibodies.

Morita YS, Jung G, Hammer JA 3rd, Fukui Y.

Eur J Cell Biol. 1996 Dec;71(4):371-9.